ArticleInfection and immunity2001
Activation of protein tyrosine kinases by Coxiella burnetii: role in actin cytoskeleton reorganization and bacterial phagocytosis.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- Review
- Review
- Modulation of the E-cadherin in human cells infected in vitro with Coxiella burnetii.PloS one · 2023Article
- Role of Goats in the Epidemiology ofBiology · 2022Review
- Role of ARP2/3 Complex-Driven Actin Polymerization in RSV Infection.Pathogens (Basel, Switzerland) · 2021Review
- Investigating mechanisms underlying genetic resistance to Salmon Rickettsial Syndrome in Atlantic salmon using RNA sequencing.BMC genomics · 2021Article
- Exosome-mediated communication between tumor cells and tumor-associated macrophages: implications for tumor microenvironment.Oncoimmunology · 2021Review
- "Hairiness" is a Facsimile of Reorganized Cytoskeletons: A Cytopathic Effect ofYonsei medical journal · 2019Review
- Article
- Coxiella burnetii Lipopolysaccharide: What Do We Know?International journal of molecular sciences · 2017Review
- Getting "Inside" Type I IFNs: Type I IFNs in Intracellular Bacterial Infections.Journal of immunology research · 2017Review
- Mechanisms of action of Coxiella burnetii effectors inferred from host-pathogen protein interactions.PloS one · 2017Article
- Cytoskeleton-centric protein transportation by exosomes transforms tumor-favorable macrophages.Oncotarget · 2016Article
- Right on Q: genetics begin to unravel Coxiella burnetii host cell interactions.Future microbiology · 2016Review
- Hijacking Host Cell Highways: Manipulation of the Host Actin Cytoskeleton by Obligate Intracellular Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- Molecular pathogenesis of the obligate intracellular bacterium Coxiella burnetii.Nature reviews. Microbiology · 2013Review
- Tripping on acid: trans-kingdom perspectives on biological acids in immunity and pathogenesis.PLoS pathogens · 2013Review
- Article
- Coxiella burnetii, the agent of Q fever, replicates within trophoblasts and induces a unique transcriptional response.PloS one · 2010Article
- Coxiella burnetii Nine Mile II proteins modulate gene expression of monocytic host cells during infection.BMC microbiology · 2010Article
Corrections and comments
- Expression of concern
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coxiella burnetii, the agent of Q fever, is an obligate intracellular microorganism that grows in monocytes/macrophages. The internalization of virulent organisms by monocytes is lower than that of avirulent variants and is associated with actin cytoskeleton reorganization. We studied the activation of protein tyrosine kinases (PTKs) by C. burnetii in THP-1 monocytes. Virulent organisms induced early PTK activation and the tyrosine phosphorylation of several endogenous substrates, including Hck and Lyn, two Src-related kinases. PTK activation reflects C. burnetii virulence since avirulent variants were unable to stimulate PTK. We also investigated the role of PTK activation in C. burnetii-stimulated F-actin reorganization. Tyrosine-phosphorylated proteins were colocalized with F-actin inside cell protrusions induced by C. burnetii, and PTK activity was increased in Triton X-100-insoluble fractions. In addition, lavendustin A, a PTK inhibitor, and PP1, a Src kinase inhibitor, prevented C. burnetii-induced cell protrusions and F-actin reorganization. We finally assessed the role of PTK activation in bacterial phagocytosis. Pretreatment of THP-1 cells with lavendustin A and PP1 upregulated the uptake of virulent C. burnetii but had no effect on the phagocytosis of avirulent organisms. Thus, it is likely that PTK activation by C. burnetii negatively regulates bacterial uptake by interfering with cytoskeleton organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.