ArticleInfection and immunity2001
Streptococcus parasanguis fimbria-associated adhesin fap1 is required for biofilm formation.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 107 citations in OpenAlex.
- The Salivary Microbiome and Predicted Metabolite Production Are Associated with Barrett's Esophagus and High-Grade Dysplasia or Adenocarcinoma.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2024Article
- Review
- Deciphering Streptococcal Biofilms.Microorganisms · 2020Review
- Functional Analysis of a Fibronectin Binding Protein of Streptococcus parasanguinis FW213.Current microbiology · 2020Article
- Functional Analysis of the Collagen Binding Proteins of Streptococcus parasanguinis FW213.mSphere · 2020Article
- The streptococcal multidomain fibrillar adhesin CshA has an elongated polymeric architecture.The Journal of biological chemistry · 2020Article
- Streptococcus oralis subsp.Infection and immunity · 2019Article
- The Two Distinct Types of SecA2-Dependent Export Systems.Microbiology spectrum · 2019Review
- Uncovering complex microbiome activities via metatranscriptomics during 24 hours of oral biofilm assembly and maturation.Microbiome · 2018Article
- Complete genome sequencing of three human clinical isolates of Staphylococcus caprae reveals virulence factors similar to those of S. epidermidis and S. capitis.BMC genomics · 2018Article
- Surface proteins involved in the adhesion of Streptococcus salivarius to human intestinal epithelial cells.Applied microbiology and biotechnology · 2018Article
- Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets.Infection and immunity · 2017Article
- Engineering and Dissecting the Glycosylation Pathway of a Streptococcal Serine-rich Repeat Adhesin.The Journal of biological chemistry · 2016Article
- New Helical Binding Domain Mediates a Glycosyltransferase Activity of a Bifunctional Protein.The Journal of biological chemistry · 2016Article
- Insights into bacterial protein glycosylation in human microbiota.Science China. Life sciences · 2016Review
- Biofilm formation by Streptococcus agalactiae: influence of environmental conditions and implicated virulence factors.Frontiers in cellular and infection microbiology · 2015Review
- Selective transport by SecA2: an expanding family of customized motor proteins.Biochimica et biophysica acta · 2014Review
- The highly conserved domain of unknown function 1792 has a distinct glycosyltransferase fold.Nature communications · 2014Article
- Gap2 promotes the formation of a stable protein complex required for mature Fap1 biogenesis.Journal of bacteriology · 2013Article
- The enhancement of biofilm formation in Group B streptococcal isolates at vaginal pH.Medical microbiology and immunology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The sanguis streptococci are primary colonizers of the tooth surface and thus form the foundation for the complex multiple species biofilm known as dental plaque. In addition, these bacteria can colonize native and prosthetic heart valves and are a common cause of endocarditis. Little is known about the molecular mechanisms governing multiple or single species biofilm development within this group of organisms. Using an in vitro assay for biofilm formation, we determined that (i) Streptococcus parasanguis FW213 can form biofilms on inert surfaces such as polystyrene and (ii) environmental and nutritional factors, such as glucose, affect S. parasanguis biofilm formation. Several isogenic mutants of FW213 were tested in the biofilm assay. Strains containing mutations in fap1, a gene encoding a protein required for assembly of fimbriae, were deficient in biofilm formation. Mutants defective in recA, PepO endopeptidase activity, or the production of a fimbriae-associated protein, FimA, were still capable of biofilm formation. Phase-contrast microscopy was used to follow biofilm development by wild-type and fap1 mutant strains on plastic coverslips over time. Wild-type FW213 attached to the surface, formed aggregates of cells, and eventually formed a dense layer of cells that included microcolonies. In contrast, few fap1 mutant cells were observed attached to the surface, and no cell aggregates or microcolonies were formed. These results suggest that the long peritrichous fimbriae of FW213 are critical for the formation of biofilms on solid surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.