ArticleInfection and immunity2001
Identification of stress-responsive genes in Streptococcus mutans by differential display reverse transcription-PCR.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- tRNA Modification Landscapes in Streptococci: Shared Losses and Clade-Specific Adaptations.bioRxiv : the preprint server for biology · 2025Article
- Environmental Control of Queuosine Levels in Streptococcus mutans tRNAs.Molecular microbiology · 2025Article
- PspC domain-containing protein (PCP) determines Streptococcus mutans biofilm formation through bacterial extracellular DNA release and platelet adhesion in experimental endocarditis.PLoS pathogens · 2021Article
- Identification and functional analysis of glutamine transporter inJournal of oral microbiology · 2020Article
- The Streptococcus mutans aminotransferase encoded by ilvE is regulated by CodY and CcpA.Journal of bacteriology · 2013Article
- Article
- The branched-chain amino acid aminotransferase encoded by ilvE is involved in acid tolerance in Streptococcus mutans.Journal of bacteriology · 2012Article
- Downregulation of GbpB, a component of the VicRK regulon, affects biofilm formation and cell surface characteristics of Streptococcus mutans.Infection and immunity · 2011Article
- Point-of-care salivary microbial tests for detection of cariogenic species--clinical relevance thereof--review.Folia microbiologica · 2010Review
- Role of GlnR in acid-mediated repression of genes encoding proteins involved in glutamine and glutamate metabolism in Streptococcus mutans.Applied and environmental microbiology · 2010Article
- ClpP of Streptococcus mutans differentially regulates expression of genomic islands, mutacin production, and antibiotic tolerance.Journal of bacteriology · 2010Article
- Characterization of a glutamate transporter operon, glnQHMP, in Streptococcus mutans and its role in acid tolerance.Journal of bacteriology · 2010Article
- Inter-specific interactions between carbon-limited soil bacteria affect behavior and gene expression.Microbial ecology · 2009Article
- Influences of capsule on cell shape and chain formation of wild-type and pcsB mutants of serotype 2 Streptococcus pneumoniae.Journal of bacteriology · 2009Article
- Development and optimization of a fluorescent differential display PCR system for analyzing the stress response in Lactobacillus sakei strains.Nutrients · 2009Article
- RNA arbitrarily primed PCR and fourier transform infrared spectroscopy reveal plasticity in the acid tolerance response of Streptococcus macedonicus.Applied and environmental microbiology · 2008Article
- Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae.Journal of bacteriology · 2007Article
- Localization of PcsB of Streptococcus pneumoniae and its differential expression in response to stress.Journal of bacteriology · 2007Article
- Evidence that accumulation of mutants in a biofilm reflects natural selection rather than stress-induced adaptive mutation.Applied and environmental microbiology · 2007Article
- Functional analysis of glucan binding protein B from Streptococcus mutans.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus mutans, which causes dental caries in the human oral cavity and occasionally causes infective endocarditis in the heart, withstands adverse environmental stress through diverse alterations in protein synthesis. Differential gene expression in response to environmental stress was analyzed by RNA fingerprinting using arbitrarily primed PCR with a panel of 11mer primers designed for differential display in Enterobacteriaceae. Dot and Northern blot hybridization confirmed that the transcription of several genes was up- or down-regulated following exposure to acid shock from pH 7.5 to 5.5. RNA of a gene designated AP-185 (acid-stress protein) was induced specifically by acid treatment, while RNA of GSP-781 (general-stress protein) was up-regulated significantly when bacteria were exposed to high osmolarity and temperature, as well as low pH. The deduced amino acid sequence of AP-185 shares homology (78% identity) with branched-chain amino acid aminotransferase. Cloning and sequence analysis of GSP-781 revealed a potential secreted protein of a molecular mass of about 43 kDa and with a pI predicted to be 5.5. Transcriptional levels of another gene, designated AR-186 (acid-repressed protein), which encodes putative aconitase, were repressed by acid treatment but were enhanced by plasma or serum components. Analogous results were identified in icd and citZ genes, and repression of these genes, along with AR-186, was also observed when they were exposed to high osmolarity and temperature. These results indicate that differential regulation of specific genes at the transcriptional level is triggered by different stress and that genes responsible for glutamate biosynthesis in the citrate pathway are coordinately regulated during the stress response of S. mutans.
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