ArticleEMBO reports2001
Regulation of intracellular trafficking of the EGF receptor by Rab5 in the absence of phosphatidylinositol 3-kinase activity.
Article in EMBO reports, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Transport mechanisms between the endocytic, recycling, and biosynthetic pathways via endosomes and theFrontiers in cell and developmental biology · 2024Review
- An iron-dependent metabolic vulnerability underlies VPS34-dependence in RKO cancer cells.PloS one · 2020Article
- Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway.PloS one · 2020Article
- Article
- A Pan-GTPase Inhibitor as a Molecular Probe.PloS one · 2015Article
- Plac8 links oncogenic mutations to regulation of autophagy and is critical to pancreatic cancer progression.Cell reports · 2014Article
- Engagement of the small GTPase Rab31 protein and its effector, early endosome antigen 1, is important for trafficking of the ligand-bound epidermal growth factor receptor from the early to the late endosome.The Journal of biological chemistry · 2014Article
- Proteomic analysis of temporally stimulated ovarian cancer cells for biomarker discovery.Molecular & cellular proteomics : MCP · 2013Article
- Phosphatidylinositol 3-kinase and Rab5 GTPase inversely regulate the Smad anchor for receptor activation (SARA) protein independently of transforming growth factor-β1.The Journal of biological chemistry · 2012Article
- Effects of photodynamic therapy on the endocytic pathway.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2011Article
- Nano rare-earth oxides induced size-dependent vacuolization: an independent pathway from autophagy.International journal of nanomedicine · 2010Article
- Myxoma and vaccinia viruses exploit different mechanisms to enter and infect human cancer cells.Virology · 2010Article
- Modulation of host cell endocytosis by the type III cytotoxin, Pseudomonas ExoS.Traffic (Copenhagen, Denmark) · 2008Article
- Akt binds to and phosphorylates phospholipase C-gamma1 in response to epidermal growth factor.Molecular biology of the cell · 2006Article
- The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane.Molecular biology of the cell · 2005Article
- Repeated cycles of rapid actin assembly and disassembly on epithelial cell phagosomes.Molecular biology of the cell · 2004Article
- Endosomal signaling of epidermal growth factor receptor stimulates signal transduction pathways leading to cell survival.Molecular and cellular biology · 2002Article
- Differential PI 3-kinase dependence of early and late phases of recycling of the internalized AT1 angiotensin receptor.The Journal of cell biology · 2002Article
- Novel mechanism for regulation of epidermal growth factor receptor endocytosis revealed by protein kinase A inhibition.Molecular biology of the cell · 2002Article
- Regulation of epidermal growth factor receptor endocytosis by wortmannin through activation of Rab5 rather than inhibition of phosphatidylinositol 3-kinase.EMBO reports · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rab5 and phosphatidylinositol 3-kinase (PI3K) have been proposed to co-regulate receptor endocytosis by controlling early endosome fusion. However, in this report we demonstrate that inhibition of epidermal growth factor (EGF)-stimulated PI3K activity by expression of the kinase-deficient PI3K p110 subunit (p110delta kin) does not block the lysosomal targeting and degradation of the EGF receptor (EGFR). Moreover, inhibition of total PI3K activity by wortmannin or LY294002 significantly enlarges EGFR-containing endosomes and dissociates the early-endosomal autoantigen EEA1 from membrane fractions. However, this does not block the lysosomal targeting and degradation of EGFR. In contrast, transfection of cells with mutant Rab5 S34N or microinjection of anti-Rabaptin5 antibodies inhibits EGFR endocytosis. Our results, therefore, demonstrate that PI3K is not universally required for the regulation of receptor intracellular trafficking. The present work suggests that the intracellular trafficking of EGFR is controlled by a novel endosome fusion pathway that is regulated by Rab5 in the absence of PI3K, rather than by the previously defined endosome fusion pathway that is co-regulated by Rab5 and PI3K.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.