ArticleTransgenic research2001
The frequency of silencing in Arabidopsis thaliana varies highly between progeny of siblings and can be influenced by environmental factors.
Article in Transgenic research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- RNA silencing in the life cycle of soybean: multiple restriction systems and spatiotemporal variation associated with plant architecture.Transgenic research · 2017Article
- Phosphorus starvation induces post-transcriptional CHS gene silencing in Petunia corolla.Plant cell reports · 2013Article
- Article
- Coincident sequence-specific RNA degradation of linked transgenes in the plant genome.Plant molecular biology · 2012Article
- Ubiquitin promoter-terminator cassette promotes genetically stable expression of the taste-modifying protein miraculin in transgenic lettuce.Plant cell reports · 2011Article
- Light intensity affects RNA silencing of a transgene in Nicotiana benthamiana plants.BMC plant biology · 2010Article
- Developmentally early and late onset of Rps10 silencing in Arabidopsis thaliana: genetic and environmental regulation.Journal of experimental botany · 2009Article
- Improved drought tolerance without undesired side effects in transgenic plants producing trehalose.Plant molecular biology · 2007Article
- Nature of stress and transgene locus influences transgene expression stability in barley.Plant molecular biology · 2006Article
- The Quest to Understand the Basis and Mechanisms that Control Expression of Introduced Transgenes in Crop Plants.Plant signaling & behavior · 2006Article
- Translation initiation by non-AUG codons in Arabidopsis thaliana transgenic plants.Plant cell reports · 2006Article
- Seed 1-cysteine peroxiredoxin antioxidants are not involved in dormancy, but contribute to inhibition of germination during stress.Plant physiology · 2003Article
- ABI3 mediates expression of the peroxiredoxin antioxidant AtPER1 gene and induction by oxidative stress.Plant molecular biology · 2003Article
- The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing.The Plant cell · 2003Article
- Analyses of single-copy Arabidopsis T-DNA-transformed lines show that the presence of vector backbone sequences, short inverted repeats and DNA methylation is not sufficient or necessary for the induction of transgene silencing.Nucleic acids research · 2002Article
- Suppressors of transcriptional transgenic silencing in Chlamydomonas are sensitive to DNA-damaging agents and reactivate transposable elements.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a collection of 111 transgenic Arabidopsis thaliana lines, silencing of the nptII gene was observed in 62 (56%) of the lines and three distinct nptII-silencing phenotypes were identified. Two T-DNA constructs were used, which differed in distance and orientation of the marker gene relative to the border sequences. Comparison of the sets of lines generated with each vector, indicate that the T-DNA construct configuration influence the incidence of lines displaying silencing, as well as the distribution of silencing phenotypes. Twenty lines were investigated more thoroughly. The frequency of silencing varied between siblings in 19 lines, including three lines containing a single T-DNA copy. The last line showed 100% silencing. The gus gene present in both constructs could be expressed in the presence of a silenced nptII gene. Investigation of methylation at a single site in the pnos promoter revealed partial methylation in multi-copy lines, but no methylation in single-copy lines. For 16 lines, the overall frequencies of silencing differed significantly between control plants and plants exposed to temperature stress; in 11 of these lines at the 0.1% level. In several cases, the frequency of silencing in progeny of stress-treated plants was higher than for the control group, while other lines showed higher frequencies of kanamycin-resistant progeny for the stress-treated sibling plants.
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