ArticleThe Plant cell2001
Novel filaments 5 nm in diameter constitute the cytosolic ring of the plastid division apparatus.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 82 citations in OpenAlex.
- Review
- Insights into the Mechanisms of Chloroplast Division.International journal of molecular sciences · 2018Review
- Divide and shape: an endosymbiont in action.Planta · 2013Review
- Mechanisms of organelle division and inheritance and their implications regarding the origin of eukaryotic cells.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2010Review
- Plastid division.AoB PLANTS · 2010Article
- The PLASTID DIVISION1 and 2 components of the chloroplast division machinery determine the rate of chloroplast division in land plant cell differentiation.The Plant cell · 2009Article
- Article
- Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga Cyanidioschyzon merolae.DNA research : an international journal for rapid publication of reports on genes and genomes · 2009Article
- Genome analysis and its significance in four unicellular algae, Cyanidioschyzon [corrected] merolae, Ostreococcus tauri, Chlamydomonas reinhardtii, and Thalassiosira pseudonana.Journal of plant research · 2008Review
- Plastid division: evolution, mechanism and complexity.Annals of botany · 2007Review
- FtsZ and the division of prokaryotic cells and organelles.Nature reviews. Molecular cell biology · 2005Review
- Origin and evolution of the chloroplast division machinery.Journal of plant research · 2005Review
- The ultrastructural features and division of secondary plastids.Journal of plant research · 2005Review
- Cell cycle-regulated, microtubule-independent organelle division in Cyanidioschyzon merolae.Molecular biology of the cell · 2005Article
- Paths toward algal genomics.Plant physiology · 2005Review
- Two types of FtsZ proteins in mitochondria and red-lineage chloroplasts: the duplication of FtsZ is implicated in endosymbiosis.Journal of molecular evolution · 2004Article
- Article
- Two Dictyostelium orthologs of the prokaryotic cell division protein FtsZ localize to mitochondria and are required for the maintenance of normal mitochondrial morphology.Eukaryotic cell · 2003Article
- ARC5, a cytosolic dynamin-like protein from plants, is part of the chloroplast division machinery.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Dynamin: the endosymbiosis ring of power?Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plastid division apparatus (called the plastid-dividing ring) has been detected in several plant and algal species at the constricted region of plastids by transmission electron microscopy. The apparatus is composed of two or three rings: an outer ring in the cytosol, an inner ring in the stroma, and a middle ring in the intermembrane space. The components of these rings are not clear. FtsZ, which forms the bacterial cytokinetic ring, has been proposed as a component of both the inner and outer rings. Here, we present the ultrastructure of the outer ring at high resolution. To visualize the outer ring by negative staining, we isolated dividing chloroplasts from a synchronized culture of a red alga, Cyanidioschyzon merolae, and lysed them with nonionic detergent Nonidet P-40. Nonidet P-40 extracted primarily stroma, thylakoids, and the inner and middle rings, leaving the envelope and outer ring largely intact. Negative staining revealed that the outer ring consists of a bundle of 5-nm filaments in which globular proteins are spaced 4.8 nm apart. Immunoblotting using an FtsZ-specific antibody failed to show immunoreactivity in the fraction containing the filament. Moreover, the filament structure and properties are unlike those of known cytoskeletal filaments. The bundle of filaments forms a very rigid structure and does not disassemble in 2 M urea. We also identified a dividing phase-specific 56-kD protein of chloroplasts as a candidate component of the ring. Our results suggest that the main architecture of the outer ring did not descend from cyanobacteria during the course of endosymbiosis but was added by the host cell early in plant evolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.