ArticleThe Plant cell2001
Gibberellin/abscisic acid antagonism in barley aleurone cells: site of action of the protein kinase PKABA1 in relation to gibberellin signaling molecules.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed, 284 citations in OpenAlex.
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- Combining transcriptomics and metabolomics to analyse the mechanism of allelopathy in Cyclachaena xanthiifolia.BMC plant biology · 2025Article
- Pan-genome analysis of the R2R3-MYB genes family inFrontiers in plant science · 2025Article
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- When Size Matters: New Insights on How Seed Size Can Contribute to the Early Stages of Plant Development.Plants (Basel, Switzerland) · 2024Review
- RGA1 regulates grain size, rice quality and seed germination in the small and round grain mutant srg5.BMC plant biology · 2024Article
- DNA methylation and lipid metabolism are involved in GA-induced maize aleurone layers PCD as revealed by transcriptome analysis.BMC plant biology · 2023Article
- Overexpression of 9-International journal of molecular sciences · 2023Article
- Transcriptome Analysis of Rice Embryo and Endosperm during Seed Germination.International journal of molecular sciences · 2023Article
- Barley metallothionein isoforms, MT2b2 and MT4, differentially respond to photohormones in barley aleurone layer and their recombinant forms show different affinity for binding to zinc and cadmium.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Article
- Regulation of reactive oxygen species and phytohormones in osmotic stress tolerance during seed germination inFrontiers in plant science · 2023Article
- Molecular cloning and characterization of an alpha-amylase inhibitor (TkAAI) gene from Trichosanthes kirilowii Maxim.Biotechnology letters · 2022Article
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- Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA).Planta · 2022Article
- Brassinosteroids regulate rice seed germination through the BZR1-RAmy3D transcriptional module.Plant physiology · 2022Article
- Genome sequencing-based coverage analyses facilitate high-resolution detection of deletions linked to phenotypes of gamma-irradiated wheat mutants.BMC genomics · 2022Article
- Genome-wide association mapping and gene expression analysis identifyFrontiers in plant science · 2022Article
- Late-maturity α-amylase (LMA): exploring the underlying mechanisms and end-use quality effects in wheat.Planta · 2021Review
48 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The antagonism between gibberellins (GA) and abscisic acid (ABA) is an important factor regulating the developmental transition from embryogenesis to seed germination. In barley aleurone layers, the expression of genes encoding alpha-amylases and proteases is induced by GA but suppressed by ABA. It has been shown that an ABA-induced protein kinase, PKABA1, mediates the ABA suppression of alpha-amylase expression. Using a barley aleurone transient expression system, we have now localized the site of action of PKABA1 relative to other signal transduction components governing the expression of alpha-amylase. The expression of alpha-amylase can be transactivated by the transcription factor GAMyb, which is itself induced by GA. A truncated GAMyb containing the DNA binding domain but lacking the transactivation domain prevents the GA induction of alpha-amylase, further supporting the notion that GAMyb mediates the GA induction of alpha-amylase expression. Although ABA and PKABA1 strongly inhibit the GA induction of alpha-amylase, they have no effect on GAMyb-transactivated alpha-amylase expression. Using a GAMyb promoter--beta-glucuronidase construct, we also show that both ABA and PKABA1 repress the GA induction of GAMyb. In the slender mutant, GAMyb and alpha-amylase are highly expressed, even in the absence of GA. However, this constitutive expression can still be inhibited by ABA, PKABA1, or an inhibitor of cGMP synthesis. On the basis of these observations, we suggest that PKABA1 acts upstream from the formation of functional GAMyb but downstream from the site of action of the Slender gene product. Because PKABA1 inhibits the GA induction of the GAMyb promoter--beta-glucuronidase construct, it appears that at least part of the action of PKABA1 is to downregulate GAMyb at the transcriptional level.
Indexed as
Identifiers
11251104PMC135510W2124053497What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.