ArticleThe Plant cell2001
A small decrease of plastid transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and phenylpropanoid metabolism.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
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116 citing papers in PubMed, 329 citations in OpenAlex.
- Review
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- Review
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- Effects of Low Light after Heading on the Yield of Direct Seeding Rice and Its Physiological Response Mechanism.Plants (Basel, Switzerland) · 2023Article
- Fine mapping and candidate gene analysis of qSB12TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Uridylation and the SKI complex orchestrate the Calvin cycle of photosynthesis through RNA surveillance ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Root-Zone COInternational journal of molecular sciences · 2022Article
- Effects of suppression of chloroplast phosphoglycerate kinase on photosynthesis in rice.Photosynthesis research · 2022Article
- Transcriptome analysis provides new ideas for studying the regulation of glucose-induced lignin biosynthesis in pear calli.BMC plant biology · 2022Article
- Enhanced Carbonylation of Photosynthetic and Glycolytic Proteins in Antibiotic Timentin-Treated Tobacco In Vitro Shoot Culture.Plants (Basel, Switzerland) · 2022Article
- Point mutations that boost aromatic amino acid production and COScience advances · 2022Article
- Biogenic Volatile Organic Compounds and Protein Expressions ofPlants (Basel, Switzerland) · 2022Article
- Suppression of chloroplast triose phosphate isomerase evokes inorganic phosphate-limited photosynthesis in rice.Plant physiology · 2022Article
56 more citing papers are in PubMed but not listed here.
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Transketolase (TK) catalyzes reactions in the Calvin cycle and the oxidative pentose phosphate pathway (OPPP) and produces erythrose-4-phosphate, which is a precursor for the shikimate pathway leading to phenylpropanoid metabolism. To investigate the consequences of decreased TK expression for primary and secondary metabolism, we transformed tobacco with a construct containing an antisense TK sequence. The results were as follows: (1) a 20 to 40% reduction of TK activity inhibited ribulose-1,5-bisphosphate regeneration and photosynthesis. The inhibition of photosynthesis became greater as irradiance increased across the range experienced in growth conditions (170 to 700 micromol m(-2) sec(-1)). TK almost completely limited the maximum rate of photosynthesis in saturating light and saturating CO(2). (2) Decreased expression of TK led to a preferential decrease of sugars, whereas starch remained high until photosynthesis was strongly inhibited. One of the substrates of TK (fructose-6-phosphate) is the starting point for starch synthesis, and one of the products (erythrose-4-phosphate) inhibits phosphoglucose isomerase, which catalyzes the first reaction leading to starch. (3) A 20 to 50% decrease of TK activity led to decreased levels of aromatic amino acids and decreased levels of the intermediates (caffeic acid and hydroxycinnamic acids) and products (chlorogenic acid, tocopherol, and lignin) of phenylpropanoid metabolism. (4) There was local loss of chlorophyll and carotene on the midrib when TK activity was inhibited by >50%, spreading onto minor veins and lamina in severely affected transformants. (5) OPPP activity was not strongly inhibited by decreased TK activity. These results identify TK activity as an important determinant of photosynthetic and phenylpropanoid metabolism and show that the provision of precursors by primary metabolism colimits flux into the shikimate pathway and phenylpropanoid metabolism.
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