ArticleThe Plant cell2001
Identification of a Ca(2+)-pectate binding site on an apoplastic peroxidase.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Oryza sativa PECTIN DEFECTIVE TAPETUM1 affects anther development through a pectin-mediated signaling pathway in rice.Plant physiology · 2022Article
- Induction of Low-Level Hydrogen Peroxide Generation by Unbleached Cotton Nonwovens as Potential Wound Dressing Materials.Journal of functional biomaterials · 2017Article
- Extra-Cellular But Extra-Ordinarily Important for Cells: Apoplastic Reactive Oxygen Species Metabolism.Frontiers in plant science · 2017Review
- Review
- Cell wall proteome of sugarcane stems: comparison of a destructive and a non-destructive extraction method showed differences in glycoside hydrolases and peroxidases.BMC plant biology · 2016Article
- Investigating Biochemical and Developmental Dependencies of Lignification with a Click-Compatible Monolignol Analog in Arabidopsis thaliana Stems.Frontiers in plant science · 2016Article
- FaPOD27 functions in the metabolism of polyphenols in strawberry fruit (Fragaria sp.).Frontiers in plant science · 2014Article
- First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement.PloS one · 2014Article
- Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties.Biotechnology for biofuels · 2013Article
- Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties.PloS one · 2013Article
- Plant cell wall proteomics: the leadership of Arabidopsis thaliana.Frontiers in plant science · 2013Article
- Towards uncovering the roles of switchgrass peroxidases in plant processes.Frontiers in plant science · 2013Article
- Calcium promotes activity and confers heat stability on plant peroxidases.Plant signaling & behavior · 2012Article
- Temporal regulation of cell-wall pectin methylesterase and peroxidase isoforms in cadmium-treated flax hypocotyl.Annals of botany · 2009Article
- In vivo cell wall loosening by hydroxyl radicals during cress seed germination and elongation growth.Plant physiology · 2009Article
- An anionic class III peroxidase from zucchini may regulate hypocotyl elongation through its auxin oxidase activity.Planta · 2009Article
- A class III peroxidase specifically expressed in pathogen-attacked barley epidermis contributes to basal resistance.Molecular plant pathology · 2008Article
- Transgenic expression of a fungal endo-polygalacturonase increases plant resistance to pathogens and reduces auxin sensitivity.Plant physiology · 2008Article
- Post-translational modifications of the basic peroxidase isoenzyme from Zinnia elegans.Plant molecular biology · 2007Article
- Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine.Plant physiology · 2006Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An apoplastic isoperoxidase from zucchini (APRX) was shown to bind strongly to polygalacturonic acid in their Ca(2)+-induced conformation. By homology modeling, we were able to identify a motif of four clustered arginines (positions 117, 262, 268, and 271) that could be responsible for this binding. To verify the role of these arginine residues in the binding process, we prepared three mutants of APRX (M1, R117S; M2, R262Q/R268S; and M3, R262Q/R268S/R271Q). APRX and the three mutants were expressed as recombinant glycoproteins by the baculovirus-insect cell system. This procedure yielded four active enzymes with similar molecular masses that were tested for their ability to bind Ca(2)+-pectate. Recombinant wild-type APRX exhibited an affinity for the pectic structure comparable to that of the native plant isoperoxidase. The mutations impaired binding depending on the number of arginine residues that were replaced. M1 and M2 showed intermediate affinities, whereas M3 did not bind at all. This was demonstrated using an in vitro binding test and on cell walls of hypocotyl cross-sections. It can be concluded that APRX bears a Ca(2)+-pectate binding site formed by four clustered arginines. This site could ensure that APRX is properly positioned in cell walls, using unesterified domains of pectins as a scaffold.
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