ArticleMolecular biology of the cell2001
The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 90 citations in OpenAlex.
- Novel homozygous SPAG17 variants cause human male infertility through multiple morphological abnormalities of spermatozoal flagella related to axonemal microtubule doublets.Asian journal of andrology · 2025Article
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- Cryo-EM structure of an active central apparatus.Nature structural & molecular biology · 2022Article
- Structural organization of the C1b projection within the ciliary central apparatus.Journal of cell science · 2021Article
- Composition and function of the C1b/C1f region in the ciliary central apparatus.Scientific reports · 2021Article
- Central Apparatus, the Molecular Kickstarter of Ciliary and Flagellar Nanomachines.International journal of molecular sciences · 2021Review
- Motile cilia genetics and cell biology: big results from little mice.Cellular and molecular life sciences : CMLS · 2021Review
- Structure of the radial spoke head and insights into its role in mechanoregulation of ciliary beating.Nature structural & molecular biology · 2021Article
- Analysis of the sperm flagellar axoneme using gene-modified mice.Experimental animals · 2020Review
- A novel hypomorphic allele ofDisease models & mechanisms · 2020Article
- The unity and diversity of the ciliary central apparatus.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Identification and mapping of central pair proteins by proteomic analysis.Biophysics and physicobiology · 2020Article
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus.The Journal of cell biology · 2019Article
- FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia.Molecular biology of the cell · 2019Article
- Review
- Proteome of the central apparatus of a ciliary axoneme.The Journal of cell biology · 2019Article
- A splice donor variant in CCDC189 is associated with asthenospermia in Nordic Red dairy cattle.BMC genomics · 2019Article
- SPAG17 Is Required for Male Germ Cell Differentiation and Fertility.International journal of molecular sciences · 2018Article
- The Central Apparatus of Cilia and Eukaryotic Flagella.Cold Spring Harbor perspectives in biology · 2017Review
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Efficient motility of the eukaryotic flagellum requires precise temporal and spatial control of its constituent dynein motors. The central pair and its associated structures have been implicated as important members of a signal transduction cascade that ultimately regulates dynein arm activity. To identify central pair components involved in this process, we characterized a Chlamydomonas motility mutant (pf6-2) obtained by insertional mutagenesis. pf6-2 flagella twitch ineffectively and lack the 1a projection on the C1 microtubule of the central pair. Transformation with constructs containing a full-length, wild-type copy of the PF6 gene rescues the functional, structural, and biochemical defects associated with the pf6 mutation. Sequence analysis indicates that the PF6 gene encodes a large polypeptide that contains numerous alanine-rich, proline-rich, and basic domains and has limited homology to an expressed sequence tag derived from a human testis cDNA library. Biochemical analysis of an epitope-tagged PF6 construct demonstrates that the PF6 polypeptide is an axonemal component that cosediments at 12.6S with several other polypeptides. The PF6 protein appears to be an essential component required for assembly of some of these polypeptides into the C1-1a projection.
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