ArticleMolecular biology of the cell2001
The nuclear export receptor Xpo1p forms distinct complexes with NES transport substrates and the yeast Ran binding protein 1 (Yrb1p).
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Exploration of the proxiOME of large subunit ribosomal proteins reveals Acl1 and Bcl1 as cooperating dedicated chaperones of Rpl1.Nucleic acids research · 2026Article
- Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals.eLife · 2019Article
- Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.Nature communications · 2018Article
- Bcp1 Is the Nuclear Chaperone of Rpl23 in Saccharomyces cerevisiae.The Journal of biological chemistry · 2017Article
- Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation.eLife · 2016Article
- The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes.Nature communications · 2016Article
- Article
- Components and regulation of nuclear transport processes.The FEBS journal · 2015Review
- Separate responses of karyopherins to glucose and amino acid availability regulate nucleocytoplasmic transport.Molecular biology of the cell · 2014Article
- A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.eLife · 2014Article
- Atomic basis of CRM1-cargo recognition, release and inhibition.Seminars in cancer biology · 2014Review
- Degradation of specific nuclear proteins occurs in the cytoplasm in Saccharomyces cerevisiae.Genetics · 2014Article
- Sumoylation regulates Kap114-mediated nuclear transport.The EMBO journal · 2012Article
- Ran-dependent nuclear export mediators: a structural perspective.The EMBO journal · 2011Review
- An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1.The EMBO journal · 2010Article
- Yeast Mpk1 cell wall integrity mitogen-activated protein kinase regulates nucleocytoplasmic shuttling of the Swi6 transcriptional regulator.Molecular biology of the cell · 2010Article
- Nap1 and Chz1 have separate Htz1 nuclear import and assembly functions.Traffic (Copenhagen, Denmark) · 2010Article
- Utp9p facilitates Msn5p-mediated nuclear reexport of retrograded tRNAs in Saccharomyces cerevisiae.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Xpo1p (Crm1p) is the nuclear export receptor for proteins containing a leucine-rich nuclear export signal (NES). Xpo1p, the NES-containing protein, and GTP-bound Ran form a complex in the nucleus that translocates across the nuclear pore. We have identified Yrb1p as the major Xpo1p-binding protein in Saccharomyces cerevisiae extracts in the presence of GTP-bound Gsp1p (yeast Ran). Yrb1p is cytoplasmic at steady-state but shuttles continuously between the cytoplasm and the nucleus. Nuclear import of Yrb1p is mediated by two separate nuclear targeting signals. Export from the nucleus requires Xpo1p, but Yrb1p does not contain a leucine-rich NES. Instead, the interaction of Yrb1p with Xpo1p is mediated by Gsp1p-GTP. This novel type of export complex requires the acidic C-terminus of Gsp1p, which is dispensable for the binding to importin beta-like transport receptors. A similar complex with Xpo1p and Gsp1p-GTP can be formed by Yrb2p, a relative of Yrb1p predominantly located in the nucleus. Yrb1p also functions as a disassembly factor for NES/Xpo1p/Gsp1p-GTP complexes by displacing the NES protein from Xpo1p/Gsp1p. This Yrb1p/Xpo1p/Gsp1p complex is then completely dissociated after GTP hydrolysis catalyzed by the cytoplasmic GTPase activating protein Rna1p.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.