ArticleThe Journal of physiology2001
Effects of sarcomere length and temperature on the rate of ATP utilisation by rabbit psoas muscle fibres.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 47 citations in OpenAlex.
- Temperature affects the torque-velocity relationship of the ankle plantar flexor muscles.Scientific reports · 2025Article
- The elementary step that generates force and sinusoidal analysis in striated muscle fibers.Journal of muscle research and cell motility · 2025Review
- American Society of Biomechanics Journal of Biomechanics Award 2021: Exploring the Functional Boundaries and Metabolic Consequences of Triceps Surae Force-Length Relations during Walking.Journal of biomechanics · 2023Article
- Shorter muscle fascicle operating lengths increase the metabolic cost of cyclic force production.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Article
- Adding carbon fiber to shoe soles may not improve running economy: a muscle-level explanation.Scientific reports · 2020Article
- Ultrasound imaging links soleus muscle neuromechanics and energetics during human walking with elastic ankle exoskeletons.Scientific reports · 2020Article
- Modular footwear that partially offsets downhill or uphill grades minimizes the metabolic cost of human walking.Royal Society open science · 2020Article
- Exhaustion of Skeletal Muscle Fibers Within Seconds: Incorporating Phosphate Kinetics Into a Hill-Type Model.Frontiers in physiology · 2020Article
- The force-length-velocity potential of the human soleus muscle is related to the energetic cost of running.Proceedings. Biological sciences · 2019Article
- Enhanced Development of Skeletal Myotubes from Porcine Induced Pluripotent Stem Cells.Scientific reports · 2017Article
- Cross-bridge kinetics in myofibrils containing familial hypertrophic cardiomyopathy R58Q mutation in the regulatory light chain of myosin.Journal of theoretical biology · 2011Article
- Effect of phosphate and temperature on force exerted by white muscle fibres from dogfish.Journal of muscle research and cell motility · 2010Article
- Observing cycling of a few cross-bridges during isometric contraction of skeletal muscle.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Synchronous in situ ATPase activity, mechanics, and Ca2+ sensitivity of human and porcine myocardium.Biophysical journal · 2009Article
- Time course and strain dependence of ADP release during contraction of permeabilized skeletal muscle fibers.Biophysical journal · 2009Article
- Two-state model of acto-myosin attachment-detachment predicts C-process of sinusoidal analysis.Biophysical journal · 2007Article
- Effect of strain on actomyosin kinetics in isometric muscle fibers.Biophysical journal · 2006Article
- The ATP hydrolysis and phosphate release steps control the time course of force development in rabbit skeletal muscle.The Journal of physiology · 2005Article
- Using optical tweezers to relate the chemical and mechanical cross-bridge cycles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Mechanokinetics of rapid tension recovery in muscle: the Myosin working stroke is followed by a slower release of phosphate.Biophysical journal · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
1. The steady state rate of ATP utilisation by single permeabilised fibres from rabbit psoas muscle immersed in silicone oil was measured using a linked enzyme assay that coupled ADP production to the oxidation of NADH.2. At sarcomere length 2.5 microm, at 10 degrees C, the rate of ATP utilisation in relaxing conditions was 6 +/- 1 microM s-1 (mean +/- S.E.M., n = 8 fibres); during isometric contraction it was 310 +/- 10 microM s-1 (mean +/- S.E.M., n = 11). Assuming a myosin active site concentration of 150 microM, these values correspond to rates of ATP utilisation per active site of about 0.04 and 2.1 s-1, respectively. 3. The rate of ATP utilisation in relaxing conditions was independent of sarcomere length in the range 2.5-4.0 microm. The rate of ATP utilisation during isometric contraction had a dependence on resting sarcomere length similar to that of isometric force in the range 2.5-4.0 microm, but was less strongly dependent on sarcomere length than was isometric force in the range 1.5-2.5 microm. 4. The rate of ATP utilisation in relaxing conditions had a Q10 of 2.5 in the temperature range 7-25 degrees C, but this increased to 9.7 in the range 25-35 degrees C, suggesting that some active force may have been generated in relaxing solution at temperatures above 25 degrees C. 5. The rate of ATP utilisation during isometric contraction had a Q10 of 3.6 throughout the temperature range 7-25 degrees C; this was similar to the Q10 for isometric force at low temperature (3.5 at 7-10 degrees C) but much larger than that for isometric force at higher temperature (1.3 at 20-25 degrees C). 6. Application of the NADH-linked assay to single muscle fibres in oil improves the effective sensitivity and time resolution of the method, and allows continuous measurements of the rate of ADP production during active contraction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.