ArticleBritish journal of pharmacology2001
Inhibitory effect of 2,3-butanedione monoxime (BDM) on Na(+)/Ca(2+) exchange current in guinea-pig cardiac ventricular myocytes.
Article in British journal of pharmacology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 64 citations in OpenAlex.
- A novel method to extend viability and functionality of living heart slices.Frontiers in cardiovascular medicine · 2023Article
- The effective use of blebbistatin to study the action potential of cardiac pacemaker cells of zebrafish (Current research in physiology · 2022Article
- Early changes in myocyte contractility and cardiac function in streptozotocin-induced type 1 diabetes in rats.PloS one · 2020Article
- Effect of pralidoxime on coronary perfusion pressure during cardiopulmonary resuscitation in a pig model.Clinical and experimental emergency medicine · 2019Article
- Eliminating contraction during culture maintains global and local CaCell calcium · 2019Article
- Acetylcholine Delays Atrial Activation to Facilitate Atrial Fibrillation.Frontiers in physiology · 2019Article
- Cardiac metabolic effects of KFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo.Journal of visualized experiments : JoVE · 2018Article
- Murine Short Axis Ventricular Heart Slices for Electrophysiological Studies.Journal of visualized experiments : JoVE · 2017Article
- Inhibitory effect of YM-244769, a novel NaNaunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Nicorandil stimulates a Na⁺/Ca²⁺ exchanger by activating guanylate cyclase in guinea pig cardiac myocytes.Pflugers Archiv : European journal of physiology · 2016Article
- Article
- Neuroprotection Against Diisopropylfluorophosphate in Acute Hippocampal Slices.Neurochemical research · 2015Article
- The role of Na+/Ca2+ exchanger subtypes in neuronal ischemic injury.Translational stroke research · 2015Review
- Analysis of tubular membrane networks in cardiac myocytes from atria and ventricles.Journal of visualized experiments : JoVE · 2014Article
- The mechanical uncoupler blebbistatin is associated with significant electrophysiological effects in the isolated rabbit heart.Experimental physiology · 2013Article
- The role of dynamic instability and wavelength in arrhythmia maintenance as revealed by panoramic imaging with blebbistatin vs. 2,3-butanedione monoxime.American journal of physiology. Heart and circulatory physiology · 2012Article
- Methods in cardiomyocyte isolation, culture, and gene transfer.Journal of molecular and cellular cardiology · 2011Review
- Regional increase of extracellular potassium leads to electrical instability and reentry occurrence through the spatial heterogeneity of APD restitution.American journal of physiology. Heart and circulatory physiology · 2011Article
- Structure and dynamics of an Arp2/3 complex-independent component of the lamellipodial actin network.Cell motility and the cytoskeleton · 2009Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The effect of 2,3-butanedione monoxime (BDM), a 'chemical phosphatase', on Na(+)/Ca(2+) exchange current (I(NCX)) was investigated using the whole-cell voltage-clamp technique in single guinea-pig cardiac ventricular myocytes and in CCL39 fibroblast cells expressing canine NCX1. 2. I(NCX) was identified as a current sensitive to KB-R7943, a relatively selective NCX inhibitor, at 140 mM Na(+) and 2 mM Ca(2+) in the external solution and 20 mM Na(+) and 433 nM free Ca(2+) in the pipette solution. 3. In guinea-pig ventricular cells, BDM inhibited I(NCX) in a concentration-dependent manner. The IC(50) value was 2.4 mM with a Hill coefficients of 1. The average time for 50% inhibition by 10 mM BDM was 124+/-31 s (n=5). 4. The effect of BDM was not affected by 1 microM okadaic acid in the pipette solution, indicating that the inhibition was not via activation of okadaic acid-sensitive protein phosphatases. 5. Intracellular trypsin treatment via the pipette solution significantly suppressed the inhibitory effect of BDM, implicating an intracellular site of action of BDM. 6. PAM (pralidoxime), another oxime compound, also inhibited I(NCX) in a manner similar to BDM. 7. Isoprenaline at 50 microM and phorbol 12-myristate 13-acetate (PMA) at 8 microM did not reverse the inhibition of I(NCX) by BDM. 8. BDM inhibited I(NCX) in CCL39 cells expressing NCX1 and in its mutant in which its three major phosphorylatable serine residues were replaced with alanines. 9. We conclude that BDM inhibits I(NCX) but the mechanism of inhibition is not by dephosphorylation of the Na(+)/Ca(2+) exchanger as a 'chemical phosphatase'.
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