ArticleProceedings of the National Academy of Sciences of the United States of America2001
Cloning and mitochondrial localization of full-length D-AKAP2, a protein kinase A anchoring protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 100 citations in OpenAlex.
- Subcellular Stress Markers in Epithelial Ovarian Cancer.International journal of molecular sciences · 2025Review
- Nanodomain cAMP signaling in cardiac pathophysiology: potential for developing targeted therapeutic interventions.Physiological reviews · 2025Review
- Phosphatases Control the Duration and Range of cAMP/PKA Microdomains.Function (Oxford, England) · 2025Article
- Mitochondrial Kinase Signaling for Cardioprotection.International journal of molecular sciences · 2024Review
- PDE-Mediated Cyclic Nucleotide Compartmentation in Vascular Smooth Muscle Cells: From Basic to a Clinical Perspective.Journal of cardiovascular development and disease · 2021Review
- Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.Physiological reports · 2021Article
- Review
- Subcellular Organization of the cAMP Signaling Pathway.Pharmacological reviews · 2021Review
- The interaction of the mitochondrial protein importer TOMM34 with HSP70 is regulated by TOMM34 phosphorylation and binding to 14-3-3 adaptors.The Journal of biological chemistry · 2020Article
- Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis.Nature communications · 2019Article
- Article
- Role of protein kinase A in regulating mitochondrial function and neuronal development: implications to neurodegenerative diseases.Reviews in the neurosciences · 2015Review
- Role of soluble adenylyl cyclase in mitochondria.Biochimica et biophysica acta · 2014Review
- cAMP signaling in subcellular compartments.Pharmacology & therapeutics · 2014Review
- A-kinase anchoring proteins 10 expression in relation to 2073A/G polymorphism and tumor progression in patients with colorectal cancer.Pathology oncology research : POR · 2013Article
- cAMP and mitochondria.Physiology (Bethesda, Md.) · 2013Review
- Article
- Creating order from chaos: cellular regulation by kinase anchoring.Annual review of pharmacology and toxicology · 2013Review
- Possible counter effect in newborns of 1936A>G (I646V) polymorphism in the AKAP10 gene encoding A-kinase-anchoring protein 10.Journal of perinatology : official journal of the California Perinatal Association · 2012Article
- Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C.BMC cell biology · 2011Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Differential compartmentalization of signaling molecules in cells and tissues is being recognized as an important mechanism for regulating the specificity of signal transduction pathways. A kinase anchoring proteins (AKAPs) direct the subcellular localization of protein kinase A (PKA) by binding to its regulatory (R) subunits. Dual specific AKAPs (D-AKAPs) interact with both RI and RII. A 372-residue fragment of mouse D-AKAP2 with a 40-residue C-terminal PKA binding region and a putative regulator of G protein signaling (RGS) domain was previously identified by means of a yeast two-hybrid screen. Here, we report the cloning of full-length human D-AKAP2 (662 residues) with an additional putative RGS domain, and the corresponding mouse protein less the first two exons (617 residues). Expression of D-AKAP2 was characterized by using mouse tissue extracts. Full-length D-AKAP2 from various tissues shows different molecular weights, possibly because of alternative splicing or posttranslational modifications. The cloned human gene product has a molecular weight similar to one of the prominent mouse proteins. In vivo association of D-AKAP2 with PKA in mouse brain was demonstrated by using cAMP agarose pull-down assay. Subcellular localization for endogenous mouse, rat, and human D-AKAP2 was determined by immunocytochemistry, immunohistochemistry, and tissue fractionation. D-AKAP2 from all three species is highly enriched in mitochondria. The mitochondrial localization and the presence of RGS domains in D-AKAP2 may have important implications for its function in PKA and G protein signal transduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.