ArticleProceedings of the National Academy of Sciences of the United States of America2001
The nucleoporin Nup98 associates with the intranuclear filamentous protein network of TPR.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 95 citations in OpenAlex.
- SARS-CoV-2 Orf6 is positioned in the nuclear pore complex by Rae1 to inhibit nucleocytoplasmic transport.Molecular biology of the cell · 2024Article
- TPR is a prognostic biomarker and potential therapeutic target associated with immune infiltration in hepatocellular carcinoma.Molecular and clinical oncology · 2024Article
- Review
- Correct dosage of X chromosome transcription is controlled by a nuclear pore component.Cell reports · 2021Article
- Article
- The Nuclear Pore Complex and mRNA Export in Cancer.Cancers · 2020Review
- The Quest for the Blueprint of the Nuclear Pore Complex.The protein journal · 2019Review
- Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution.Cellular and molecular life sciences : CMLS · 2019Article
- Tpr regulates the total number of nuclear pore complexes per cell nucleus.Genes & development · 2018Article
- HIV integration sites and implications for maintenance of the reservoir.Current opinion in HIV and AIDS · 2018Review
- Nucleoporins redistribute inside the nucleus after cell cycle arrest induced by histone deacetylases inhibition.Nucleus (Austin, Tex.) · 2017Article
- Subnuclear positioning and interchromosomal clustering of the GAL1-10 locus are controlled by separable, interdependent mechanisms.Molecular biology of the cell · 2016Article
- SINC, a type III secreted protein of Chlamydia psittaci, targets the inner nuclear membrane of infected cells and uninfected neighbors.Molecular biology of the cell · 2015Article
- Chromatin organization at the nuclear pore favours HIV replication.Nature communications · 2015Article
- Components and regulation of nuclear transport processes.The FEBS journal · 2015Review
- Sperm and spermatids contain different proteins and bind distinct egg factors.International journal of molecular sciences · 2014Article
- Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function.Journal of cell science · 2014Article
- Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity.Molecular biology of the cell · 2014Article
- Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP.Nucleic acids research · 2014Article
- Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Nup98 gene codes for several alternatively spliced protein precursors. Two in vitro translated and autoproteolytically cleaved precursors yielded heterodimers of Nup98-6kDa peptide and Nup98-Nup96. TPR (translocated promoter region) is a protein that forms filamentous structures extending from nuclear pore complexes (NPCs) to intranuclear sites. We found that in vitro translated TPR bound to in vitro translated Nup98 and, via Nup98, to Nup96. Double-immunofluorescence microscopy with antibodies to TPR and Nup98 showed colocalization. In confocal sections the nucleolus itself was only weakly stained but there was intensive perinucleolar staining. Striking spike-like structures emanated from this perinucleolar ring and attenuated into thinner structures as they extended to the nuclear periphery. This characteristic staining pattern of the TPR network was considerably enhanced when a myc-tagged pyruvate kinase-6kDa fusion protein was overexpressed in HeLa cells. Double-immunoelectron microscopy of these cells using anti-myc and anti-TPR antibodies and secondary gold-coupled antibodies yielded row-like arrangements of gold particles. Taken together, the immunolocalization data support previous electron microscopical data, suggesting that TPR forms filaments that extend from the NPC to the nucleolus. We discuss the possible implications of the association of Nup98 with this intranuclear TPR network for an intranuclear phase of transport.
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