ArticleProceedings of the National Academy of Sciences of the United States of America2001
Human fatty acid synthase: role of interdomain in the formation of catalytically active synthase dimer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 94 citations in OpenAlex.
- The implications of FASN in viral infection and related diseases: a promising target in antiviral therapies.Frontiers in cellular and infection microbiology · 2026Review
- The role of gene duplication and paralog specialisation in the evolution of the mammalian PRPS complex.Nature communications · 2025Article
- PRPS activity tunes redox homeostasis in Myc-driven lymphoma.Redox biology · 2025Article
- Identification ofGenes · 2024Article
- Microbial ensemble in the hives: deciphering the intricate gut ecosystem of hive and forager bees of Apis mellifera.Molecular biology reports · 2024Article
- Exposure to dietary fatty acids oleic and palmitic acid alters structure and mechanotransduction of intestinal cells in vitro.Archives of toxicology · 2023Article
- Malonyl/Acetyltransferase (MAT) Knockout Decreases Triacylglycerol and Medium-Chain Fatty Acid Contents in Goat Mammary Epithelial Cells.Foods (Basel, Switzerland) · 2022Article
- Prevalence of elevated serum fatty acid synthase in chronic limb-threatening ischemia.Scientific reports · 2021Observational
- Similarities and Differences: A Comparative Review of the Molecular Mechanisms and Effectors of NAFLD and AFLD.Frontiers in physiology · 2021Review
- Metabolism.Essays in biochemistry · 2020Review
- Flavonones fromInternational journal of molecular sciences · 2018Article
- Neuronal Lipid Metabolism: Multiple Pathways Driving Functional Outcomes in Health and Disease.Frontiers in molecular neuroscience · 2018Review
- GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells.BioMed research international · 2017Article
- S-nitrosylation of fatty acid synthase regulates its activity through dimerization.Journal of lipid research · 2016Article
- Targeting FASN in Breast Cancer and the Discovery of Promising Inhibitors from Natural Products Derived from Traditional Chinese Medicine.Evidence-based complementary and alternative medicine : eCAM · 2014Review
- Article
- Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- Identification of a new fatty acid synthesis-transport machinery at the peroxisomal membrane.The Journal of biological chemistry · 2012Article
- Crystal structure and catalytic properties of Bacillus anthracis CoADR-RHD: implications for flavin-linked sulfur trafficking.Biochemistry · 2009Article
- The 2.7-Angstrom crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
The human and animal fatty acid synthases are dimers of two identical multifunctional proteins (M(r) 272,000) arranged in an antiparallel configuration. This arrangement generates two active centers for fatty acid synthesis separated by interdomain (ID) regions and predicts that two appropriate halves of the monomer should be able to reconstitute an active fatty acid synthesizing center. This prediction was confirmed by the reconstitution of the synthase active center by using two heterologously expressed halves of the monomer protein. Each of these recombinant halves of synthase monomer contains half of the ID regions. We show here that the fatty acid synthase activity could not be reconstituted when the ID sequences present in the two recombinant halves are deleted, suggesting that these ID sequences are essential for fatty acid synthase dimer formation. Further, we confirm that the ID sequences are the only regions of fatty acid synthase monomers that showed significant dimer formation, by using the yeast two-hybrid system. These results are consistent with the proposal that the ID region, which has no known catalytic activity, associates readily and holds together the two dynamic active centers of the fatty acid synthase dimer, therefore playing an important role in the architecture of catalytically active fatty acid synthase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.