ArticlePlant molecular biology2001
A recombinase-mediated transcriptional induction system in transgenic plants.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 98 citations in OpenAlex.
- Gene activation via Cre/lox-mediated excision in cowpea (Vigna unguiculata).Plant cell reports · 2022Article
- A memory switch for plant synthetic biology based on the phage ϕC31 integration system.Nucleic acids research · 2020Article
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- Comparison of threePlant methods · 2020Article
- Construction of Marker-Free Genetically Modified Maize Using a Heat-Inducible Auto-Excision Vector.Genes · 2019Article
- Heat-Shock-Induced Removal of Transgenes Using the Gene-Deletor System in Hybrid Aspen (Genes · 2018Article
- A new location to split Cre recombinase for protein fragment complementation.Plant biotechnology journal · 2017Article
- Synthetic Botany.Cold Spring Harbor perspectives in biology · 2017Review
- Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi.Transgenic research · 2016Article
- Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDDIranian journal of biotechnology · 2015Article
- Article
- Excision of a selectable marker gene in transgenic banana using a Cre/lox system controlled by an embryo specific promoter.Plant molecular biology · 2013Article
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Development of a simple and efficient system for excising selectable markers in Arabidopsis using a minimal promoter::Cre fusion construct.Molecules and cells · 2012Article
- Key proliferative activity in the junction between the leaf blade and leaf petiole of Arabidopsis.Plant physiology · 2011Article
- Heat-inducible Cre-lox system for marker excision in transgenic rice.Journal of biosciences · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- Selectable antibiotic resistance marker gene-free transgenic rice harbouring the garlic leaf lectin gene exhibits resistance to sap-sucking planthoppers.Plant cell reports · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We constructed and tested a Cre-loxP recombination-mediated vector system termed pCrox for use in transgenic plants. In this system, treatment of Arabidopsis under inducing conditions mediates an excision event that removes an intervening piece of DNA between a promoter and the gene to be expressed. The system developed here uses a heat-shock-inducible Cre to excise a DNA fragment flanked by lox sites, thereby generating a constitutive GUS reporter gene under control of the CaMV 35S promoter. Heat-shock-mediated excision of several, independent lines resulted in varying degrees of recombination-mediated GUS activation. Induction was shown to be possible at essentially any stage of plant growth. This single vector system circumvents the need for genetic crosses required by other, dual recombinase vector systems. The pCrox system may prove particularly useful in instances where transgene over-expression, or under-expression by antisense, would otherwise affect embryo, seed or seedling viability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.