ArticlePlant molecular biology2001
Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- pBAR-H3.2, a native-optimized binary vector to bypass transgene silencing in alfalfa.Plant cell reports · 2020Article
- Genome-Wide Identification and Characterization of UTR-Introns ofInternational journal of molecular sciences · 2020Article
- Intron-Mediated Enhancement: A Tool for Heterologous Gene Expression in Plants?Frontiers in plant science · 2016Review
- Phylogeny, structural evolution and functional diversification of the plant PHOSPHATE1 gene family: a focus on Glycine max.BMC evolutionary biology · 2013Article
- The 5'UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis.BMC plant biology · 2012Article
- Molecular analysis of T-DNA insertion mutants identified putative regulatory elements in the AtTERT gene.Journal of experimental botany · 2011Article
- A transmembrane domain containing pellicle protein of Toxoplasma gondii enhances virulence and invasion after extracellular stress.Molecular and biochemical parasitology · 2011Article
- DNA free energy-based promoter prediction and comparative analysis of Arabidopsis and rice genomes.Plant physiology · 2011Article
- Testing the IMEter on rice introns and other aspects of intron-mediated enhancement of gene expression.Journal of experimental botany · 2011Article
- Histone3 variants in plants.Chromosoma · 2010Review
- Activity of the 5' regulatory regions of the rice polyubiquitin rubi3 gene in transgenic rice plants as analyzed by both GUS and GFP reporter genes.Plant cell reports · 2008Article
- Gene expression enhancement mediated by the 5' UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants.Molecular genetics and genomics : MGG · 2008Article
- Promoter-proximal introns in Arabidopsis thaliana are enriched in dispersed signals that elevate gene expression.The Plant cell · 2008Article
- Identification of a dehydration and ABA-responsive promoter regulon and isolation of corresponding DNA binding proteins for the group 4 LEA gene CpC2 from C. plantagineum.Plant molecular biology · 2006Article
- Constitutive expression exposes functional redundancy between the Arabidopsis histone H2A gene HTA1 and other H2A gene family members.The Plant cell · 2006Article
- Effect of 5'UTR introns on gene expression in Arabidopsis thaliana.BMC genomics · 2006Article
- Article
- Identification of Arabidopsis rat mutants.Plant physiology · 2003Article
- Splicing of the maize Sh1 first intron is essential for enhancement of gene expression, and a T-rich motif increases expression without affecting splicing.Plant physiology · 2002Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intron-bearing replacement histone H3 genes in Arabidopsis and other plants are highly and constitutively expressed. We demonstrate that the introns located within the 5'-untranslated regions (5'-UTR) of the two Arabidopsis replacement H3 genes will abolish the cell cycle dependence of an endogenous histone H4 promoter. We demonstrate that these introns, functionally combined with their endogenous promoters, could produce the high and constitutive expression of the replacement H3 genes observed in planta. They strongly increase gene expression whatever the promoter, from the strong 35S CaMV promoter to complete and resected promoters of cell cycle-dependent and replacement histone genes. Quantitative analysis of the extent of reporter gene enhancement in different parts of developing transgenic plantlets, ranging from 2-fold to 70-fold, supports the notion that trans-acting factors are responsible for this effect. Such factors appear most abundant in roots.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.