ArticleJournal of bacteriology2001
Heteromeric interactions among nucleoid-associated bacterial proteins: localization of StpA-stabilizing regions in H-NS of Escherichia coli.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Article
- Rok from B. subtilis: Bridging genome structure and transcription regulation.Molecular microbiology · 2025Review
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Molecular and thermodynamic determinants of self-assembly and hetero-oligomerization in the enterobacterial thermo-osmo-regulatory protein H-NS.Nucleic acids research · 2024Article
- The environmentally-regulated interplay between local three-dimensional chromatin organisation and transcription of proVWX in E. coli.Nature communications · 2023Article
- Organization of the bacterial nucleoid by DNA-bridging proteins and globular crowders.Frontiers in microbiology · 2023Article
- Article
- Effects of Global and Specific DNA-Binding Proteins on Transcriptional Regulation of theInternational journal of molecular sciences · 2022Article
- Histone-like nucleoid structuring (H-NS) protein silences the beta-glucoside (Computational and structural biotechnology journal · 2022Article
- Histone-like Nucleoid-Structuring Protein (H-NS) Paralogue StpA Activates the Type I-E CRISPR-Cas System against Natural Transformation in Escherichia coli.Applied and environmental microbiology · 2020Article
- Crystal structure of an Escherichia coli Hfq Core (residues 2-69)-DNA complex reveals multifunctional nucleic acid binding sites.Nucleic acids research · 2020Article
- Chromosome organization in bacteria: mechanistic insights into genome structure and function.Nature reviews. Genetics · 2020Review
- Review
- Ancestral zinc-finger bearing protein MucR in alpha-proteobacteria: A novel xenogeneic silencer?Computational and structural biotechnology journal · 2020Review
- The architects of bacterial DNA bridges: a structurally and functionally conserved family of proteins.Open biology · 2019Article
- StpA and Hha stimulate pausing by RNA polymerase by promoting DNA-DNA bridging of H-NS filaments.Nucleic acids research · 2018Article
- Molecular basis for the differential expression of the global regulator VieA in Vibrio cholerae biotypes directed by H-NS, LeuO and quorum sensing.Molecular microbiology · 2018Article
- H-NS Regulates Gene Expression and Compacts the Nucleoid: Insights from Single-Molecule Experiments.Biophysical journal · 2015Review
- Silencing by H-NS potentiated the evolution of Salmonella.PLoS pathogens · 2014Article
- Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleoid-associated proteins H-NS and StpA in Escherichia coli bind DNA as oligomers and are implicated in gene regulatory systems. There is evidence for both homomeric and heteromeric H-NS-StpA complexes. The two proteins show differential turnover, and StpA was previously found to be subject to protease-mediated degradation by the Lon protease. We investigated which regions of the H-NS protein are able to prevent degradation of StpA. A set of truncated H-NS derivatives was tested for their ability to mediate StpA stability and to form heteromers in vitro. The data indicate that H-NS interacts with StpA at two regions and that the presence of at least one of the H-NS regions is necessary for StpA stability. Our results also suggest that a proteolytically stable form of StpA, StpA(F21C), forms dimers, whereas wild-type StpA in the absence of H-NS predominantly forms tetramers or oligomers, which are more susceptible to proteolysis.
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