ArticleNucleic acids research2001
The dhp1(+) gene, encoding a putative nuclear 5'-->3' exoribonuclease, is required for proper chromosome segregation in fission yeast.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Observation of conformational changes that underlie the catalytic cycle of Xrn2.Nature chemical biology · 2022Article
- Two plasmid modules for introducing the auxin-inducible degron into the fission yeastmicroPublication biology · 2021Article
- Hypomodified tRNA in evolutionarily distant yeasts can trigger rapid tRNA decay to activate the general amino acid control response, but with different consequences.PLoS genetics · 2020Article
- A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin.eLife · 2020Article
- Condensin locates at transcriptional termination sites in mitosis, possibly releasing mitotic transcripts.Open biology · 2019Article
- IT'S 2 for the price of 1: Multifaceted ITS2 processing machines in RNA and DNA maintenance.DNA repair · 2019Review
- A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5'-3' Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe.PLoS genetics · 2016Article
- Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- mRNA stability in the nucleus.Journal of Zhejiang University. Science. B · 2014Review
- lncRNA recruits RNAi and the exosome to dynamically regulate pho1 expression in response to phosphate levels in fission yeast.Genes & development · 2014Article
- Normal and Aberrantly Capped mRNA Decapping.The Enzymes · 2012Article
- Rhn1, a nuclear protein, is required for suppression of meiotic mRNAs in mitotically dividing fission yeast.PloS one · 2012Article
- Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.Yeast (Chichester, England) · 2011Article
- Identification of noncoding transcripts from within CENP-A chromatin at fission yeast centromeres.The Journal of biological chemistry · 2011Article
- Structural and biochemical studies of the 5'→3' exoribonuclease Xrn1.Nature structural & molecular biology · 2011Article
- Article
- Structure and function of the 5'-->3' exoribonuclease Rat1 and its activating partner Rai1.Nature · 2009Article
- A complex gene regulatory mechanism that operates at the nexus of multiple RNA processing decisions.Nature structural & molecular biology · 2009Article
- Article
- Requirement of fission yeast Cid14 in polyadenylation of rRNAs.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Schizosaccharomyces pombe dhp1(+) gene is an ortholog of the Saccharomyces cerevisiae RAT1 gene, which encodes a nuclear 5'-->3' exoribonuclease, and is essential for cell viability. To clarify the cellular functions of the nuclear 5'-->3' exoribonuclease, we isolated and characterized a temperature-sensitive mutant of dhp1 (dhp1-1 mutant). The dhp1-1 mutant showed nuclear accumulation of poly(A)(+) RNA at the restrictive temperature, as was already reported for the rat1 mutant. Interestingly, the dhp1-1 mutant exhibited aberrant chromosome segregation at the restrictive temperature. The dhp1-1 cells frequently contained condensed chromosomes, most of whose sister chromatids failed to separate during mitosis despite normal mitotic spindle elongation. Finally, chromosomes were displaced or unequally segregated. As similar mitotic defects were also observed in Dhp1p-depleted cells, we concluded that dhp1(+) is required for proper chromosome segregation as well as for poly(A)(+) RNA metabolism in fission yeast. Furthermore, we isolated a multicopy suppressor of the dhp1-1 mutant, referred to as din1(+). We found that the gene product of dhp1-1 was unstable at high temperatures, but that reduced levels of Dhp1-1p could be suppressed by overexpressing Din1p at the restrictive temperature. Thus, Din1p may physically interact with Dhp1p and stabilize Dhp1p and/or restore its activity.
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