ArticleNucleic acids research2001
Gene2EST: a BLAST2 server for searching expressed sequence tag (EST) databases with eukaryotic gene-sized queries.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Prospects of genomic resources available at the global databases for the flora of United Arab Emirates.3 Biotech · 2019Article
- Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression.The Journal of biological chemistry · 2011Article
- Quantitative gene expression profiles in real time from expressed sequence tag databases.Gene expression · 2010Article
- Systematic genome-wide annotation of spliceosomal proteins reveals differential gene family expansion.Genome research · 2006Article
- EST-BASED ANALYSIS OF GENE EXPRESSION IN THE HUMAN COCHLEA.Workshops & poster abstracts : [proceedings]. IEEE Computational Systems Bioinformatics Conference · 2005Article
- Sequence variability, gene structure, and expression of full-length human endogenous retrovirus H.Journal of virology · 2005Article
- galaxieEST: addressing EST identity through automated phylogenetic analysis.BMC bioinformatics · 2004Article
- e2g: an interactive web-based server for efficiently mapping large EST and cDNA sets to genomic sequences.Nucleic acids research · 2004Article
- Article
- GeneSeqer@PlantGDB: Gene structure prediction in plant genomes.Nucleic acids research · 2003Article
- Genomic expansion and clustering of ZAD-containing C2H2 zinc-finger genes in Drosophila.EMBO reports · 2002Article
- Single nucleotide polymorphism mapping using genome-wide unique sequences.Genome research · 2002Article
- Comparative genomic analysis in the region of a major Plasmodium-refractoriness locus of Anopheles gambiae.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Overlapping antisense transcription in the human genome.Comparative and functional genomics · 2002Article
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4 authors.
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Abstract
Expressed sequence tags (ESTs) are randomly sequenced cDNA clones. Currently, nearly 3 million human and 2 million mouse ESTs provide valuable resources that enable researchers to investigate the products of gene expression. The EST databases have proven to be useful tools for detecting homologous genes, for exon mapping, revealing differential splicing, etc. With the increasing availability of large amounts of poorly characterised eukaryotic (notably human) genomic sequence, ESTs have now become a vital tool for gene identification, sometimes yielding the only unambiguous evidence for the existence of a gene expression product. However, BLAST-based Web servers available to the general user have not kept pace with these developments and do not provide appropriate tools for querying EST databases with large highly spliced genes, often spanning 50 000-100 000 bases or more. Here we describe Gene2EST (http://woody.embl-heidelberg.de/gene2est/), a server that brings together a set of tools enabling efficient retrieval of ESTs matching large DNA queries and their subsequent analysis. RepeatMasker is used to mask dispersed repetitive sequences (such as Alu elements) in the query, BLAST2 for searching EST databases and Artemis for graphical display of the findings. Gene2EST combines these components into a Web resource targeted at the researcher who wishes to study one or a few genes to a high level of detail.
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