ArticleNucleic acids research2001
A link between DNA methylation and epigenetic silencing in transgenic Volvox carteri.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Genome-wide adenine N6-methylation map reveals epigenomic regulation of lipid accumulation in Nannochloropsis.Plant communications · 2024Article
- Light Dependent Changes in Adenylate Methylation of the Promoter of the Mitochondrial Citrate Synthase Gene in Maize (International journal of molecular sciences · 2022Article
- N6-methyladenine: A Rare and Dynamic DNA Mark.Advances in experimental medicine and biology · 2022Article
- The epigenetic origin of life history transitions in plants and algae.Plant reproduction · 2021Review
- DNA NPlants (Basel, Switzerland) · 2020Review
- Inhibition of DNA Methylation inFrontiers in genetics · 2020Article
- Volvox and volvocine green algae.EvoDevo · 2020Review
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus.BMC biotechnology · 2019Article
- UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems.Plant molecular biology · 2016Article
- N6-Methyladenine: A Conserved and Dynamic DNA Mark.Advances in experimental medicine and biology · 2016Review
- Dynamic Changes in the Transcriptome and Methylome of Chlamydomonas reinhardtii throughout Its Life Cycle.Plant physiology · 2015Article
- Article
- Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.Genetics · 2008Article
- Maintenance DNA methyltransferase (Met1) and silencing of CpG-methylated foreign DNA in Volvox carteri.Plant molecular biology · 2007Article
- SET3p monomethylates histone H3 on lysine 9 and is required for the silencing of tandemly repeated transgenes in Chlamydomonas.Nucleic acids research · 2007Article
- Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis.Applied and environmental microbiology · 2006Article
- Analysis and accurate quantification of CpG methylation by MALDI mass spectrometry.Nucleic acids research · 2003Article
- Methylation of adenine in the nuclear DNA of Tetrahymena is internucleosomal and independent of histone H1.Nucleic acids research · 2002Article
- Suppressors of transcriptional transgenic silencing in Chlamydomonas are sensitive to DNA-damaging agents and reactivate transposable elements.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic silencing of foreign genes introduced into plants poses an unsolved problem for transgenic technology. Here we have used the simple multicellular green alga VOLVOX: carteri as a model to analyse the relation of DNA methylation to transgenic silencing. VOLVOX: DNA contains on average 1.1% 5-methylcytosine and 0.3% N6-methyladenine, as revealed by electrospray mass spectrometry and phosphoimaging of chromatographically separated (32)P-labelled nucleotides. In two nuclear transformants of V.carteri, produced in 1993 by biolistic bombardment with a foreign arylsulphatase gene (C-ars), the transgene is still expressed in one (Hill 181), but not in the other (Hill 183), after an estimated 500-1000 generations. Each transformant clone contains multiple intact copies of C-ars, most of them integrated into the genome as tandem repeats. When the bisulphite genomic sequencing protocol was applied to examine two select regions of transgenic C-ars, we found that the inactivated copies (Hill 183) exhibited a high-level methylation (40%) of CpG dinucleotides, whereas the active copies (Hill 181) displayed low-level (7%) CpG methylation. These are average values from 40 PCR clones sequenced from each DNA strand in the two portions of C-ars. The observed correlation of CpG methylation and transgene inactivation in a green alga will be discussed in the light of transcriptional silencing.
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