ArticleMolecular and cellular biology2001
A truncated form of the human CAF-1 p150 subunit impairs the maintenance of transcriptional gene silencing in mammalian cells.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1.International journal of molecular sciences · 2022Article
- CAF-1/p150 promotes cell proliferation, migration, invasion and predicts a poor prognosis in patients with cervical cancer.Oncology letters · 2020Article
- The histone chaperone CAF-1 cooperates with the DNA methyltransferases to maintainGenes & development · 2019Article
- CHAF1A interacts with TCF4 to promote gastric carcinogenesis via upregulation of c-MYC and CCND1 expression.EBioMedicine · 2018Article
- HP1 cooperates with CAF-1 to compact heterochromatic transgene repeats in mammalian cells.Scientific reports · 2018Article
- Overexpression of chromatin assembly factor-1/p60 predicts biological behaviour of laryngeal carcinomas.Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale · 2017Article
- Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma.OncoTargets and therapy · 2016Article
- Cell-cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei.Nucleic acids research · 2012Article
- Epigenetic disregulation in oral cancer.International journal of molecular sciences · 2012Review
- Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance.Genetics · 2011Article
- Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites.DNA repair · 2009Article
- Control of trichome branching by chromatin assembly factor-1.BMC plant biology · 2008Article
- E2F regulates FASCIATA1, a chromatin assembly gene whose loss switches on the endocycle and activates gene expression by changing the epigenetic status.Plant physiology · 2007Article
- Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells.Molecular biology of the cell · 2007Article
- The chromatin assembly factor subunit FASCIATA1 is involved in homologous recombination in plants.The Plant cell · 2006Article
- Heat shock-independent induction of multidrug resistance by heat shock factor 1.Molecular and cellular biology · 2006Article
- RelA repression of RelB activity induces selective gene activation downstream of TNF receptors.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Silencing of chromatin assembly factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis.Molecular and cellular biology · 2004Article
- BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.Genes & development · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin assembly factor 1 (CAF-1) is a protein complex formed of three subunits, p150, p60, and p48, conserved from the yeast Saccharomyces cerevisiae to humans, which can promote nucleosome assembly onto newly replicated DNA. In S. cerevisiae, deletion of the genes encoding any of the three CAF-1 subunits (cacDelta mutants), although nonlethal, results in a silencing defect of genes packaged into heterochromatin. Here we report on a mammalian cell model that we devised to monitor gene silencing and its reversal in a quantitative manner. This model relies on the use of a cell line stably transfected with a reporter gene in a silenced state. Reversal of reporter gene silencing was achieved upon treatment of the cells with 5-azacytidine, which resulted in the demethylation of the reporter gene copies. We show that expression of a cDNA for the human p150 CAF-1 subunit harboring 5' truncations, but not that of a cDNA encoding the full-length p150 CAF-1 subunit, increases by more than 500-fold the frequency at which transcriptional silencing of the reporter gene copies is reversed in these cells. Reversal of gene silencing is dependent upon expression of a truncated protein, possibly acting as a dominant negative mutant of the wild-type CAF-1, is associated with alterations in chromatin structure as measured by an endonuclease sensitivity assay and is not associated with detectable changes in the methylation status of the silenced genes. These results suggest that the role of CAF-1 in the epigenetic control of gene expression has been conserved between yeast and mammals, despite the lack of DNA methylation in yeast chromatin.
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