ArticleMolecular and cellular biology2001
Role of the 3' splice site in U12-dependent intron splicing.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Minor Intron Splicing from Basic Science to Disease.International journal of molecular sciences · 2021Review
- New insights into minor splicing-a transcriptomic analysis of cells derived from TALS patients.RNA (New York, N.Y.) · 2019Article
- Minor intron splicing revisited: identification of new minor intron-containing genes and tissue-dependent retention and alternative splicing of minor introns.BMC genomics · 2019Article
- Most human introns are recognized via multiple and tissue-specific branchpoints.Genes & development · 2018Article
- Consideration of non-canonical splice sites improves gene prediction on the Arabidopsis thaliana Niederzenz-1 genome sequence.BMC research notes · 2017Article
- Large-scale analysis of branchpoint usage across species and cell lines.Genome research · 2017Article
- Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density maps.RNA biology · 2016Article
- U6atac snRNA stem-loop interacts with U12 p65 RNA binding protein and is functionally interchangeable with the U12 apical stem-loop III.Scientific reports · 2016Article
- U12-type spliceosomal introns of Insecta.International journal of biological sciences · 2012Article
- The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing.Genes & development · 2010Article
- The conserved 3' end domain of U6atac snRNA can direct U6 snRNA to the minor spliceosome.RNA (New York, N.Y.) · 2009Article
- Mutational analysis of the U12-dependent branch site consensus sequence.RNA (New York, N.Y.) · 2008Article
- Alternative splicing and bioinformatic analysis of human U12-type introns.Nucleic acids research · 2007Article
- A mutational analysis of U12-dependent splice site dinucleotides.RNA (New York, N.Y.) · 2005Article
- Comparison of splice sites in mammals and chicken.Genome research · 2005Article
- Both U2 snRNA and U12 snRNA are required for accurate splicing of exon 5 of the rat calcitonin/CGRP gene.RNA (New York, N.Y.) · 2004Article
- Determinants of plant U12-dependent intron splicing efficiency.The Plant cell · 2004Article
- Identification, characterization and molecular phylogeny of U12-dependent introns in the Arabidopsis thaliana genome.Nucleic acids research · 2003Article
- Branchpoint selection in the splicing of U12-dependent introns in vitro.RNA (New York, N.Y.) · 2002Article
- A computational scan for U12-dependent introns in the human genome sequence.Nucleic acids research · 2001Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
U12-dependent introns containing alterations of the 3' splice site AC dinucleotide or alterations in the spacing between the branch site and the 3' splice site were examined for their effects on splice site selection in vivo and in vitro. Using an intron with a 5' splice site AU dinucleotide, any nucleotide could serve as the 3'-terminal nucleotide, although a C residue was most active, while a U residue was least active. The penultimate A residue, by contrast, was essential for 3' splice site function. A branch site-to-3' splice site spacing of less than 10 or more than 20 nucleotides strongly activated alternative 3' splice sites. A strong preference for a spacing of about 12 nucleotides was observed. The combined in vivo and in vitro results suggest that the branch site is recognized in the absence of an active 3' splice site but that formation of the prespliceosomal complex A requires an active 3' splice site. Furthermore, the U12-type spliceosome appears to be unable to scan for a distal 3' splice site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.