ArticleMolecular and cellular biology2001
The balance of nuclear import and export determines the intracellular distribution and function of tomato heat stress transcription factor HsfA2.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed.
- Identification of a natural RcLOG1 allele linked to prickle development in Rose (Rosa spp.).Planta · 2025Article
- Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors inPlants (Basel, Switzerland) · 2025Article
- Genome-wide characterization of theFrontiers in plant science · 2025Article
- Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family inInternational journal of molecular sciences · 2024Article
- Genome-wide analysis of the HSF family in Allium sativum L. and AsHSFB1 overexpression in Arabidopsis under heat stress.BMC genomics · 2024Article
- Genome-wide identification, phylogeny and expression analysis of Hsf gene family in Verbena bonariensis under low-temperature stress.BMC genomics · 2024Article
- Plant Adaptation and Tolerance to Heat Stress: Advance Approaches and Future Aspects.Combinatorial chemistry & high throughput screening · 2024Review
- SCHIZORIZA domain-function analysis identifies requirements for its specific role in cell fate segregation.Plant physiology · 2023Article
- Characterization of the Heat Shock Transcription Factor Family inInternational journal of molecular sciences · 2023Article
- Ectopic overexpression of TaHsfA5 promotes thermomorphogenesis in Arabidopsis thaliana and thermotolerance in Oryza sativa.Plant molecular biology · 2023Article
- Genome-Wide Characterization and Expression of theInternational journal of molecular sciences · 2023Article
- Genome-wide identification of the heat shock transcription factor gene family in two kiwifruit species.Frontiers in plant science · 2023Article
- Hsf transcription factor gene family in peanut (Frontiers in plant science · 2023Article
- Genome-wide analysis of HSF family and overexpression ofFrontiers in plant science · 2023Article
- Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Frontiers in plant science · 2023Article
- Genome-wide analysis of the heat shock transcription factor family reveals saline-alkali stress responses inPeerJ · 2023Article
- Regulatory Mechanisms of Heat Stress Response and Thermomorphogenesis in Plants.Plants (Basel, Switzerland) · 2022Review
- Elucidating the functional role of heat stress transcription factor A6b (TaHsfA6b) in linking heat stress response and the unfolded protein response in wheat.Plant molecular biology · 2022Article
- Characteristics and Regulating Roles of Wheat TaHsfA2-13 in Abiotic Stresses.Frontiers in plant science · 2022Article
- Molecular insights into mechanisms underlying thermo-tolerance in tomato.Frontiers in plant science · 2022Review
17 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
Tomato heat stress transcription factor HsfA2 is a shuttling protein with dominant cytoplasmic localization as a result of a nuclear import combined with an efficient export. Besides the nuclear localization signal (NLS) adjacent to the oligomerization domain, a C-terminal leucine-rich motif functions as a nuclear export signal (NES). Mutant forms of HsfA2 with a defective or an absent NES are nuclear proteins. The same is true for the wild-type HsfA2 if coexpressed with HsfA1 or in the presence of export inhibitor leptomycin B (LMB). Fusion of the NES domain of HsfA2 to HsfB1, which is a nuclear protein, caused export of the HsfB1-A2NES hybrid protein, and this effect was reversed by the addition of LMB. Due to the lack of background problems, Chinese hamster ovary (CHO) cells represent an excellent system for expression and functional analysis of tomato Hsfs. The results faithfully reflect the situation found in plant cells (tobacco protoplasts). The intriguing role of NLS and NES accessibility for the intracellular distribution of HsfA2 is underlined by the results of heat stress treatments of CHO cells (41 degrees C). Despite the fact that nuclear import and export are not markedly affected, HsfA2 remains completely cytoplasmic at 41 degrees C even in the presence of LMB. The temperature-dependent conformational transition of HsfA2 with shielding of the NLS evidently needs intramolecular interaction between the internal HR-A/B and the C-terminal HR-C regions. It is not observed with the HR oligomerization domain (HR-A/B region) deletion form of HsfA2 or in HsfA2-HsfA1 hetero-oligomers.
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