ArticleMolecular and cellular biology2001
Ambient pH signaling regulates nuclear localization of the Aspergillus nidulans PacC transcription factor.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Alkaliphilic/Alkali-Tolerant Fungi: Molecular, Biochemical, and Biotechnological Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Organic acid, a virulence factor for pathogenic fungi, causing postharvest decay in fruits.Molecular plant pathology · 2022Review
- Article
- Regulation of Secondary Metabolism in theInternational journal of molecular sciences · 2020Review
- A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning.Applied microbiology and biotechnology · 2020Article
- The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes.Communications biology · 2020Article
- A Highly Efficient Xylan-Utilization System inFrontiers in microbiology · 2018Article
- Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein.Molecular microbiology · 2015Article
- Multiple nuclear localization signals mediate nuclear localization of the GATA transcription factor AreA.Eukaryotic cell · 2014Article
- The Cryptococcus neoformans Rim101 transcription factor directly regulates genes required for adaptation to the host.Molecular and cellular biology · 2014Article
- Article
- Receptor-mediated signaling in Aspergillus fumigatus.Frontiers in microbiology · 2013Article
- The Cryptococcus neoformans capsule: a sword and a shield.Clinical microbiology reviews · 2012Review
- Pho85 kinase, a cyclin-dependent kinase, regulates nuclear accumulation of the Rim101 transcription factor in the stress response of Saccharomyces cerevisiae.Eukaryotic cell · 2010Article
- Analysis of wide-domain transcriptional regulation in solid-state cultures of Aspergillus oryzae.Journal of industrial microbiology & biotechnology · 2010Article
- Interaction of Cryptococcus neoformans Rim101 and protein kinase A regulates capsule.PLoS pathogens · 2010Article
- Organization and dynamics of the Aspergillus nidulans Golgi during apical extension and mitosis.Molecular biology of the cell · 2009Article
- Roles of the pH signaling transcription factor PacC in Wangiella (Exophiala) dermatitidis.Fungal genetics and biology : FG & B · 2009Article
- Establishment of the ambient pH signaling complex in Aspergillus nidulans: PalI assists plasma membrane localization of PalH.Eukaryotic cell · 2007Article
- PalC, one of two Bro1 domain proteins in the fungal pH signalling pathway, localizes to cortical structures and binds Vps32.Traffic (Copenhagen, Denmark) · 2007Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Aspergillus nidulans zinc finger transcription factor PacC is activated by proteolytic processing in response to ambient alkaline pH. The pH-regulated step is the transition of full-length PacC from a closed to an open, protease-accessible conformation. Here we show that in the absence of ambient pH signaling, the C-terminal negative-acting domain prevents the nuclear localization of full-length closed PacC. In contrast, the processed PacC form is almost exclusively nuclear at any ambient pH. In the presence of ambient pH signaling, the fraction of PacC that is in the open conformation but has not yet been processed localizes to the nucleus. Therefore, ambient alkaline pH leads to an increase in nuclear PacC by promoting the proteolytic elimination of the negative-acting domain to yield the processed form and by increasing the proportion of full-length protein that is in the open conformation. These findings explain why mutations resulting in commitment of PacC to processing irrespective of ambient pH lead to permanent PacC activation and alkalinity mimicry. A nuclear import signal that targets Escherichia coli beta-galactosidase to the nucleus has been located to the PacC zinc finger region. A mutation abolishing DNA binding does not prevent nuclear localization of the processed form, showing that PacC processing does not lead to nuclear localization by passive diffusion of the protein made possible by the reduction in size, followed by retention in the nucleus after DNA binding.
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