ArticleMolecular and cellular biology2001
Analysis of core promoter sequences located downstream from the TATA element in the hsp70 promoter from Drosophila melanogaster.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Application of the 3C Method to Study the Developmental Genes inFrontiers in genetics · 2022Article
- Two promoters integrate multiple enhancer inputs to drive wild-type knirps expression in the Drosophila melanogaster embryo.Genetics · 2021Article
- Regulation of Promoter Proximal Pausing of RNA Polymerase II in Metazoans.Journal of molecular biology · 2021Review
- The Core Promoter Is a Regulatory Hub for Developmental Gene Expression.Frontiers in cell and developmental biology · 2021Review
- Molecular Cloning and Characterization of Small Heat Shock Protein Genes in the Invasive Leaf Miner Fly,Genes · 2019Article
- A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes.Nucleic acids research · 2017Article
- TATA boxes in gene transcription and poly (A) tails in mRNA stability: New perspective on the effects of berberine.Scientific reports · 2015Article
- ElemeNT: a computational tool for detecting core promoter elements.Transcription · 2015Article
- Precise maps of RNA polymerase reveal how promoters direct initiation and pausing.Science (New York, N.Y.) · 2013Article
- Genome-wide computational prediction and analysis of core promoter elements across plant monocots and dicots.PloS one · 2013Article
- Core promoter-selective function of HMGA1 and Mediator in Initiator-dependent transcription.Genes & development · 2011Article
- Negative elongation factor accelerates the rate at which heat shock genes are shut off by facilitating dissociation of heat shock factor.Molecular and cellular biology · 2011Article
- Promoting developmental transcription.Development (Cambridge, England) · 2010Review
- NELF and GAGA factor are linked to promoter-proximal pausing at many genes in Drosophila.Molecular and cellular biology · 2008Article
- Functional characterization of core promoter elements: the downstream core element is recognized by TAF1.Molecular and cellular biology · 2005Article
- Cloning and characterization of the human beta2-glycoprotein I (beta2-GPI) gene promoter: roles of the atypical TATA box and hepatic nuclear factor-1alpha in regulating beta2-GPI promoter activity.The Biochemical journal · 2004Article
- NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.Genes & development · 2003Article
- Phosphorylation of histone H3 during transcriptional activation depends on promoter structure.Genes & development · 2003Article
- Regulation of heat shock proteins, Hsp70 and Hsp64, in heat-shocked Malpighian tubules of Drosophila melanogaster larvae.Cell stress & chaperones · 2002Article
- GAGA factor and the TFIID complex collaborate in generating an open chromatin structure at the Drosophila melanogaster hsp26 promoter.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
TFIID recognizes multiple sequence elements in the hsp70 promoter of Drosophila. Here, we investigate the function of sequences downstream from the TATA element. A mutation in the initiator was identified that caused an eightfold reduction in binding of TFIID and a fourfold reduction in transcription in vitro. Another mutation in the +24 to +29 region was somewhat less inhibitory, but a mutation in the +14 to +19 region had essentially no effect. The normal promoter and the mutants in the initiator and the +24 to +29 region were transformed into flies by P element-mediated transformation. The initiator mutation reduced expression an average of twofold in adult flies, whereas the mutation in the +24 to +29 region had essentially no effect. In contrast, a promoter combining the two mutations was expressed an average of sixfold less than the wild type. The results suggest that the initiator and the +24 to +29 region could serve overlapping functions in vivo. Protein-DNA cross-linking was used to identify which subunits of TFIID contact the +24 to +29 region and the initiator. No specific subunits were found to cross-link to the +24 to +29 region. In contrast, the initiator cross-linked exclusively to dTAF230. Remarkably, dTAF230 cross-links approximately 10 times more efficiently to the nontranscribed strand than to the transcribed strand at the initiator.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.