ArticleMolecular and cellular biology2001
Interpretation of X chromosome dose at Sex-lethal requires non-E-box sites for the basic helix-loop-helix proteins SISB and daughterless.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Evidence That Runt Acts as a Counter-Repressor of Groucho DuringG3 (Bethesda, Md.) · 2020Article
- Daughterless homodimer synergizes with Eyeless to induce Atonal expression and retinal neuron differentiation.Developmental biology · 2014Article
- Evolution of the sex-lethal gene in insects and origin of the sex-determination system in Drosophila.Journal of molecular evolution · 2014Article
- The Drosophila Myc gene, diminutive, is a positive regulator of the Sex-lethal establishment promoter, Sxl-Pe.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- A shared enhancer controls a temporal switch between promoters during Drosophila primary sex determination.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Maternal Groucho and bHLH repressors amplify the dose-sensitive X chromosome signal in Drosophila sex determination.Developmental biology · 2008Article
- Indirect effects of ploidy suggest X chromosome dose, not the X:A ratio, signals sex in Drosophila.PLoS biology · 2007Article
- Male or female? The answer depends on when you ask.PLoS biology · 2007Article
- Repression of Na,K-ATPase beta1-subunit by the transcription factor snail in carcinoma.Molecular biology of the cell · 2004Article
- Post-transcriptional regulation of the E/Daughterless ortholog HLH-2, negative feedback, and birth order bias during the AC/VU decision in C. elegans.Genes & development · 2003Article
- Article
- Article
- RNA binding protein sex-lethal (Sxl) and control of Drosophila sex determination and dosage compensation.Microbiology and molecular biology reviews : MMBR · 2003Review
- Functional conservation of the sex-lethal sex determining promoter, Sxl-Pe, in Drosophila virilis.Development genes and evolution · 2003Article
- A molecular link between gene-specific and chromosome-wide transcriptional repression.Genes & development · 2002Article
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For Drosophila melanogaster flies, sexual fate is determined by the X chromosome number. The basic helix-loop-helix protein product of the X-linked sisterlessB (sisB or scute) gene is a key indicator of the X dose and functions to activate the switch gene Sex-lethal (Sxl) in female (XX), but not in male (XY), embryos. Zygotically expressed sisB and maternal daughterless (da) proteins are known to form heterodimers that bind E-box sites and activate transcription. We examined SISB-Da binding at Sxl by using footprinting and gel mobility shift assays and found that SISB-Da binds numerous clustered sites in the establishment promoter Sxl(Pe). Surprisingly, most SISB-Da sites at Sxl(Pe) differ from the canonical CANNTG E-box motif. These noncanonical sites have 6-bp CA(G/C)CCG and 7-bp CA(G/C)CTTG cores and exhibit a range of binding affinities. We show that the noncanonical sites can mediate SISB-Da-activated transcription in cell culture. P-element transformation experiments show that these noncanonical sites are essential for Sxl(Pe) activity in embryos. Together with previous deletion analysis, the data suggest that the number, affinity, and position of SISB-Da sites may all be important for the operation of the Sxl(Pe) switch. Comparisons with other dose-sensitive promoters suggest that threshold responses to diverse biological signals have common molecular mechanisms, with important variations tailored to suit particular functional requirements.
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