ArticleThe Journal of cell biology2001
Rop GTPase-dependent dynamics of tip-localized F-actin controls tip growth in pollen tubes.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 187 papers.
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Who cites it
187 citing papers in PubMed, 422 citations in OpenAlex.
- TipQuant: a robust algorithm for quantitative analysis of spatiotemporally dynamic activities in tip-growing cells.The New phytologist · 2026Article
- Nucleotide-dependent switching and RIPb effector recognition of the barley susceptibility factor RACB.Communications biology · 2026Article
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- A GhSnRK10-GhRopGEF5-GhROP10 signaling pathway regulates cotton fiber cell growth.Plant communications · 2025Article
- The evolution of RHO of plant (ROP) proteins and their morphogenetic functions.The New phytologist · 2025Review
- Novel small molecules disrupting polarized cell expansion and development in the moss,Plant biotechnology (Tokyo, Japan) · 2025Article
- CPK1 activates CNGCs through phosphorylation for CaNature communications · 2025Article
- Polar localization and local translation of RHO-RELATED PROTEIN FROM PLANTS2 mRNAs promote root hair growth in Arabidopsis.The Plant cell · 2024Article
- AtHMGB15 regulates tapetal apoptosis in pollen development and actin dynamics during pollen germination in arabidopsis.Plant reproduction · 2024Article
- RHO OF PLANTS signalling and the activating ROP GUANINE NUCLEOTIDE EXCHANGE FACTORS: specificity in cellular signal transduction in plants.Journal of experimental botany · 2024Review
- PbrCSP1, a pollen tube-specific cold shock domain protein, is essential for the growth and cold resistance of pear pollen tubes.Molecular breeding : new strategies in plant improvement · 2024Article
- VAMP726 and VAMP725 regulate vesicle secretion and pollen tube growth in Arabidopsis.Plant cell reports · 2023Article
- Two subtypes of GTPase-activating proteins coordinate tip growth and cell size regulation in Physcomitrium patens.Nature communications · 2023Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Integrated analysis of biparental and natural populations reveals CRIB domain-containing protein underlying seed coat crack trait in watermelon.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes.PLoS biology · 2023Article
- Signaling network controlling ROP-mediated tip growth in Arabidopsis and beyond.Plant communications · 2023Review
- ROP signaling regulates spatial pattern of cell division and specification of meristem notch.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
127 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tip-growing pollen tubes provide a useful model system to study polar growth. Although roles for tip-focused calcium gradient and tip-localized Rho-family GTPase in pollen tube growth is established, the existence and function of tip-localized F-actin have been controversial. Using the green fluorescent protein-tagged actin-binding domain of mouse talin, we found a dynamic form of tip-localized F-actin in tobacco pollen tubes, termed short actin bundles (SABs). The dynamics of SABs during polar growth in pollen tubes is regulated by Rop1At, a Rop GTPase belonging to the Rho family. When overexpressed, Rop1At transformed SAB into a network of fine filaments and induced a transverse actin band behind the tip, leading to depolarized growth. These changes were due to ectopic Rop1At localization to the apical region of the plasma membrane and were suppressed by guanine dissociation inhibitor overexpression, which removed ectopically localized Rop1At. Rop GTPase-activating protein (RopGAP1) overexpression, or Latrunculin B treatments, also recovered normal actin organization and tip growth in Rop1At-overexpressing tubes. Moreover, overexpression of RopGAP1 alone disrupted SABs and inhibited growth. Finally, SAB oscillates and appears at the tip before growth. Together, these results indicate that the dynamics of tip actin are essential for tip growth and provide the first direct evidence to link Rho GTPase to actin organization in controlling cell polarity and polar growth in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.