ArticleThe Journal of cell biology2001
Evidence for a replication function of FFA-1, the Xenopus orthologue of Werner syndrome protein.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- WRN structural flexibility showcased through fragment-based lead discovery of inhibitors.Nature communications · 2026Article
- The N-terminus of RPA large subunit and its spatial position are important for the 5'->3' resection of DNA double-strand breaks.Nucleic acids research · 2015Article
- Analysis of MRE11's function in the 5'-->3' processing of DNA double-strand breaks.Nucleic acids research · 2012Article
- Replication protein A promotes 5'-->3' end processing during homology-dependent DNA double-strand break repair.The Journal of cell biology · 2011Article
- Helicase-inactivating mutations as a basis for dominant negative phenotypes.Cell cycle (Georgetown, Tex.) · 2010Review
- Roles of Werner syndrome protein in protection of genome integrity.DNA repair · 2010Review
- RecQ helicases: guardian angels of the DNA replication fork.Chromosoma · 2008Review
- Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability.The Biochemical journal · 2006Review
- Analysis of the Xenopus Werner syndrome protein in DNA double-strand break repair.The Journal of cell biology · 2005Article
- RecQ helicases: suppressors of tumorigenesis and premature aging.The Biochemical journal · 2003Review
- The yeast Sgs1 helicase is differentially required for genomic and ribosomal DNA replication.The EMBO journal · 2003Article
- RPA is an initiation factor for human chromosomal DNA replication.Nucleic acids research · 2003Article
- Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae.Genetics · 2002Article
- Werner Syndrome.Journal of biomedicine & biotechnology · 2002Article
- Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
DNA replication in higher eukaryotic cells occurs at a large number of discrete sites called replication foci. We have previously purified a protein, focus-forming activity 1 (FFA-1), which is involved in the assembly of putative prereplication foci in Xenopus egg extracts. FFA-1 is the orthologue of the Werner syndrome gene product (WRN), a member of the RecQ helicase family. In this paper we show that FFA-1 colocalizes with sites of DNA synthesis and the single-stranded DNA binding protein, replication protein A (RPA), in nuclei reconstituted in the egg extract. In addition, we show that two glutathione S-transferase FFA-1 fusion proteins can inhibit DNA replication in a dominant negative manner. The dominant negative effect correlates with the incorporation of the fusion proteins into replication foci to form "hybrid foci," which are unable to engage in DNA replication. At the biochemical level, RPA can interact with FFA-1 and specifically stimulates its DNA helicase activity. However, in the presence of the dominant negative mutant proteins, the stimulation is prevented. These results provide the first direct biochemical evidence of an important role for FFA-1 in DNA replication.
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