ArticleGenetics2001
A targeted gene knockout in Drosophila.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- Arc controls organ architecture through modulation of Crb and MyoII.The Journal of cell biology · 2025Article
- Selection with two alleles of X-linkage and its application to the fitness component analysis of OdsH in Drosophila.G3 (Bethesda, Md.) · 2024Article
- Generation of Mutants from the 57B Region ofGenes · 2023Article
- Regulatory modules mediating the complex neural expression patterns of the homeobrain gene during Drosophila brain development.Hereditas · 2022Article
- Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting.Hereditas · 2021Article
- Hedgehog signaling and Tre1 regulate actin dynamics through PI(4,5)PCell reports · 2021Article
- The homeodomain transcription factor Orthopedia is involved in development of the Drosophila hindgut.Hereditas · 2020Article
- Cytoplasmic and Mitochondrial NADPH-Coupled Redox Systems in the Regulation of Aging.Nutrients · 2019Review
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Lateral inhibition: Two modes of non-autonomous negative autoregulation by neuralized.PLoS genetics · 2018Article
- Conservation of cardiac L-type CaJournal of molecular and cellular cardiology · 2018Article
- Deletion of endogenous Tau proteins is not detrimental in Drosophila.Scientific reports · 2016Article
- Genome Engineering with TALE and CRISPR Systems in Neuroscience.Frontiers in genetics · 2016Review
- Gene targeting and transgene stacking using intra genomic homologous recombination in plants.Plant methods · 2016Review
- Complementation between polymerase- and exonuclease-deficient mitochondrial DNA polymerase mutants in genomically engineered flies.Nature communications · 2015Article
- Article
- Drosophila sugar receptors in sweet taste perception, olfaction, and internal nutrient sensing.Current biology : CB · 2015Article
- Prestin is an anion transporter dispensable for mechanical feedback amplification in Drosophila hearing.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2015Article
- Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti.PloS one · 2015Article
- Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture.Memorias do Instituto Oswaldo Cruz · 2014Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously described a method for targeted homologous recombination at the yellow gene of Drosophila melanogaster. Because only a single gene was targeted, further work was required to show whether the method could be extended to become generally useful for gene modification in Drosophila. We have now used this method to produce a knockout of the autosomal pugilist gene by homologous recombination between the endogenous locus and a 2.5-kb DNA fragment. This was accomplished solely by tracking the altered genetic linkage of an arbitrary marker gene as the targeting DNA moved from chromosome X or 2 to chromosome 3. The results indicate that this method of homologous recombination is likely to be generally useful for Drosophila gene targeting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.