ArticleGenetics2001
The Neurospora crassa genome: cosmid libraries sorted by chromosome.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era.Mycology · 2017Article
- Article
- Characterization of the temperature-sensitive mutations un-7 and png-1 in Neurospora crassa.PloS one · 2010Article
- A tether for Woronin body inheritance is associated with evolutionary variation in organelle positioning.PLoS genetics · 2009Article
- Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting.The Journal of cell biology · 2008Article
- Two zinc-cluster transcription factors control induction of alternative oxidase in Neurospora crassa.Genetics · 2007Article
- Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like gene.Molecular genetics and genomics : MGG · 2005Article
- Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.Microbiology and molecular biology reviews : MMBR · 2004Review
- What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence.Nucleic acids research · 2003Article
- Mapping by sequencing the Pneumocystis genome using the ordering DNA sequences V3 tool.Genetics · 2003Article
- An ensemble method for identifying regulatory circuits with special reference to the qa gene cluster of Neurospora crassa.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Bacterial artificial chromosome-based physical map of the rice genome constructed by restriction fingerprint analysis.Genetics · 2001Article
- Article
- ODS2: a multiplatform software application for creating integrated physical and genetic maps.Genetics · 2001Article
- Article
- Article
- The ste3 pheromone receptor gene of Pneumocystis carinii is surrounded by a cluster of signal transduction genes.Genetics · 2001Article
- Accumulation of stress and inducer-dependent plant-cell-wall-degrading enzymes during asexual development in Aspergillus nidulans.Genetics · 2001Article
- Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Neurospora crassa cosmid library of 12,000 clones (at least nine genome equivalents) has been created using an improved cosmid vector pLorist6Xh, which contains a bacteriophage lambda origin of replication for low-copy-number replication in bacteria and the hygromycin phosphotransferase marker for direct selection in fungi. The electrophoretic karyotype of the seven chromosomes comprising the 42.9-Mb N. crassa genome was resolved using two translocation strains. Using gel-purified chromosomal DNAs as probes against the new cosmid library and the commonly used medium-copy-number pMOcosX N. crassa cosmid library in two independent screenings, the cosmids were assigned to chromosomes. Assignments of cosmids to linkage groups on the basis of the genetic map vs. the electrophoretic karyotype are 93 +/- 3% concordant. The size of each chromosome-specific subcollection of cosmids was found to be linearly proportional to the size of the particular chromosome. Sequencing of an entire cosmid containing the qa gene cluster indicated a gene density of 1 gene per 4 kbp; by extrapolation, 11,000 genes would be expected to be present in the N. crassa genome. By hybridizing 79 nonoverlapping cosmids with an average insert size of 34 kbp against cDNA arrays, the density of previously characterized expressed sequence tags (ESTs) was found to be slightly <1 per cosmid (i.e., 1 per 40 kbp), and most cosmids, on average, contained an identified N. crassa gene sequence as a starting point for gene identification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.