ArticleGenetics2001
Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03112031 (A Randomized Trial of Tamoxifen Combined With Amphotericin B and Fluconazole for Cryptococcal Meningitis), which is not on this map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Randomized Trial of Tamoxifen Combined With Amphotericin B and Fluconazole for Cryptococcal Meningitis
Who cites it
36 citing papers in PubMed, 87 citations in OpenAlex.
- A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans.Journal of microbiology (Seoul, Korea) · 2020Article
- Clinical features and cause analysis of false positive results of Aspergillus galactomannan assay in pulmonary cryptococcosis patients.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- A randomized open label trial of tamoxifen combined with amphotericin B and fluconazole for cryptococcal meningitis.Wellcome open research · 2019Article
- A chemical genetic screen reveals a role for proteostasis in capsule and biofilm formation byMicrobial cell (Graz, Austria) · 2018Article
- Vaccination with RecombinantmBio · 2017Article
- New technology and resources for cryptococcal research.Fungal genetics and biology : FG & B · 2015Review
- Pbx proteins in Cryptococcus neoformans cell wall remodeling and capsule assembly.Eukaryotic cell · 2014Article
- Identification of the galactosyltransferase of Cryptococcus neoformans involved in the biosynthesis of basidiomycete-type glycosylinositolphosphoceramide.Glycobiology · 2013Article
- Effect of virulence factors on the photodynamic inactivation of Cryptococcus neoformans.PloS one · 2013Article
- Development of a single-gene, signature-tag-based approach in combination with alanine mutagenesis to identify listeriolysin O residues critical for the in vivo survival of Listeria monocytogenes.Infection and immunity · 2012Article
- RNA interference in Cryptococcus neoformans.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Toward an integrated model of capsule regulation in Cryptococcus neoformans.PLoS pathogens · 2011Article
- Isocitrate dehydrogenase is important for nitrosative stress resistance in Cryptococcus neoformans, but oxidative stress resistance is not dependent on glucose-6-phosphate dehydrogenase.Eukaryotic cell · 2010Article
- Mixed infections and In Vivo evolution in the human fungal pathogen Cryptococcus neoformans.mBio · 2010Article
- KRE genes are required for beta-1,6-glucan synthesis, maintenance of capsule architecture and cell wall protein anchoring in Cryptococcus neoformans.Molecular microbiology · 2010Article
- A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans.The Journal of biological chemistry · 2009Article
- Identification of ENA1 as a virulence gene of the human pathogenic fungus Cryptococcus neoformans through signature-tagged insertional mutagenesis.Eukaryotic cell · 2009Article
- Evidence of a role for monocytes in dissemination and brain invasion by Cryptococcus neoformans.Infection and immunity · 2009Article
- Systematic genetic analysis of virulence in the human fungal pathogen Cryptococcus neoformans.Cell · 2008Article
- PKC1 is essential for protection against both oxidative and nitrosative stresses, cell integrity, and normal manifestation of virulence factors in the pathogenic fungus Cryptococcus neoformans.Eukaryotic cell · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cryptococcus neoformans var. neoformans is an important opportunistic fungal pathogen of patients whose immune system has been compromised due to viral infection, antineoplastic chemotherapy, or tissue transplantation. As many as 13% of all AIDS patients suffer a life-threatening cryptococcal infection at some time during the course of their HIV disease. To begin to understand the molecular basis for virulence in Cryptococcus neoformans var. neoformans serotype A, we have employed signature-tagged mutagenesis (STM) to identify mutants with altered virulence in a mouse model. The critical parameters of signature-tagged mutagenesis in C. neoformans are explored. Data are presented showing that at least 100 different strains can be mixed together in a single animal with each participating in the infection and that there is no apparent interaction between a virulent strain and an avirulent strain in our animal model. Using signature-tagged mutagenesis, we identified 39 mutants with significantly altered growth in a competitive assay. Molecular analyses of these mutants indicated that 19 (49%) contained an insertion in the actin promoter by homologous recombination from a single crossover event, creating a duplication of the actin promoter and the integration of single or multiple copies of the vector. Analysis of the chromosomal insertion sites of those mutants that did not have an integration event in the actin promoter revealed an approximately random distribution among the chromosomes. Individual challenge of the putative mutants in a mouse model revealed five hypovirulent mutants and one hypervirulent mutant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.