ArticleClinical and diagnostic laboratory immunology2001
Enzyme immunoassay detection of antigen-specific immunoglobulin g antibodies in longitudinal serum samples from patients with cryptosporidiosis.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Use of Serologic Responses against Enteropathogens to Assess the Impact of a Point-of-Use Water Filter: A Randomized Controlled Trial in Western Province, Rwanda.The American journal of tropical medicine and hygiene · 2017Trial
- Decrease in Incidence of Diarrhea Due to Cryptosporidium in Bangladeshi Children Is Associated With an Increase in Anti-Cryptosporidium Antibody Avidity.The Journal of infectious diseases · 2025Observational
- Early immune and host cell responses toFrontiers in parasitology · 2023Article
- Enteropathogen antibody dynamics and force of infection among children in low-resource settings.eLife · 2019Article
- Human Seroprevalence to 11 Zoonotic Pathogens in the U.S. Arctic, Alaska.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2019Article
- Application of a salivary immunoassay in a prospective community study of waterborne infections.Water research · 2018Observational
- Giardia and Cryptosporidium antibody prevalence and correlates of exposure among Alaska residents, 2007-2008.Epidemiology and infection · 2018Article
- Use of Pathogen-Specific Antibody Biomarkers to Estimate Waterborne Infections in Population-Based Settings.Current environmental health reports · 2016Review
- Serum Anti-Cryptosporidial gp15 Antibodies in Mothers and Children Less than 2 Years of Age in India.The American journal of tropical medicine and hygiene · 2015Article
- Rapid microfluidic immunoassay for surveillance and diagnosis of Cryptosporidium infection in human immunodeficiency virus-infected patients.Biomicrofluidics · 2015Article
- Household Socioeconomic and Demographic Correlates of Cryptosporidium Seropositivity in the United States.PLoS neglected tropical diseases · 2015Article
- Prospects for immunotherapy and vaccines against Cryptosporidium.Human vaccines & immunotherapeutics · 2014Review
- Serum IgG responses and seroconversion patterns to Cryptosporidium gp15 among children in a birth cohort in south India.Clinical and vaccine immunology : CVI · 2012Article
- Systemic antibody responses to the immunodominant p23 antigen and p23 polymorphisms in children with cryptosporidiosis in Bangladesh.The American journal of tropical medicine and hygiene · 2012Article
- Multiplex assay detection of immunoglobulin G antibodies that recognize Giardia intestinalis and Cryptosporidium parvum antigens.Clinical and vaccine immunology : CVI · 2010Article
- Cloning and characterization of the acidic ribosomal protein P2 of Cryptosporidium parvum, a new 17-kilodalton antigen.Clinical and vaccine immunology : CVI · 2010Article
- Recent diarrhea is associated with elevated salivary IgG responses to Cryptosporidium in residents of an eastern Massachusetts community.Infection · 2010Article
- Longitudinal analysis of cryptosporidium species-specific immunoglobulin G antibody responses in Peruvian children.Clinical and vaccine immunology : CVI · 2006Article
- Changes in serum immunoglobulin G levels as a marker for Cryptosporidium sp. infection in Peruvian children.Journal of clinical microbiology · 2005Article
- Quality assurance considerations in cryptosporidium antibody tests.Clinical and diagnostic laboratory immunology · 2003Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidium parvum is a protozoan parasite that causes diarrheal illness in a wide range of mammalian hosts, including humans. Characteristic serum immunoglobulin G (IgG) antibody responses to antigens in the 27- and 17-kDa size ranges have been shown to develop after infection, and several enzyme-linked immunosorbent assay (ELISA) and Western blot assay formats have been used to measure these IgG levels in human serum. Using a collection of serial samples from laboratory-confirmed cryptosporidiosis patients, we compared the results obtained by using two new ELISAs with those obtained with two different Western blot assays. When assayed with the large-format Western blot, 97% of the 67 patients had a demonstrable antibody response on at least one occasion. The Cp23 ELISA correctly identified 93% of the samples that had a 27-kDa response by Western blot and 100% of the negative samples. The Triton antigen ELISA detected 77% of the samples that had a 17-kDa response by Western blot and 88% of the negative samples. The sensitivity of the Triton antigen assay was higher for samples collected between 16 and 92 days after the onset of symptoms (96%). The minigel-format Western blot did not compare favorably with the large-format blot for the detection of antibodies to the 27-kDa antigen (71% sensitivity). A half-life of about 12 weeks was estimated for antibodies to both the 27- and 17-kDa antigens. We believe the Cp23 and Triton antigen ELISAs will be useful in epidemiologic studies of the prevalence of Cryptosporidium infection in the population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.