ArticleClinical and diagnostic laboratory immunology2001
A single amino acid change within antigenic domain II of the spike protein of bovine coronavirus confers resistance to virus neutralization.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 68 citations in OpenAlex.
- Identification of Conserved B-Cell Epitope Candidates Associated with Neutralizing Activity of Bovine Coronavirus Spike Protein.Animals : an open access journal from MDPI · 2026Article
- A Review: Bovine Coronavirus Evolution, Molecular Epidemiology, and Genetic Variation.Viruses · 2026Review
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- Genetic diversity of bovine coronaviruses from recurrent outbreaks on a large-scale cattle farm.Tropical animal health and production · 2026Article
- Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis.Veterinary research · 2026Article
- Molecular characterization of BCoV infecting vaccinated and non-vaccinated cattle in Thrace district Türkiye and isolation of field strains.Virology journal · 2025Article
- Diversifying selection identified in immune epitopes of bovine coronavirus isolates from Irish cattle.The Journal of general virology · 2024Article
- A comprehensive molecular analysis of bovine coronavirus strains isolated from Brazil and comparison of a wild-type and cell culture-adapted strain associated with respiratory disease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Article
- Characterization and Spike Gene Analysis of a Candidate Attenuated Live Bovine Coronavirus Vaccine.Animals : an open access journal from MDPI · 2024Article
- First report and genomic characterization of a bovine-like coronavirus causing enteric infection in an odd-toed non-ruminant species (Indonesian tapir, Acrocodia indica) during an outbreak of winter dysentery in a zoo.Transboundary and emerging diseases · 2022Article
- SARS-CoV-2 Infection of Rhesus Macaques Treated Early with Human COVID-19 Convalescent Plasma.Microbiology spectrum · 2021Article
- Article
- Bovine coronavirus infections in Turkey: molecular analysis of the full-length spike gene sequences of viruses from digestive and respiratory infections.Archives of virology · 2021Article
- Bovine coronavirus in neonatal calf diarrhoea in Iran.Veterinary medicine and science · 2020Article
- First detection and genomic characteristics of bovine torovirus in dairy calves in China.Archives of virology · 2020Article
- Molecular epidemiological survey and phylogenetic analysis of bovine respiratory coronavirus in Japan from 2016 to 2018.The Journal of veterinary medical science · 2020Article
- Genomic Characterization and Phylogenetic Classification of Bovine Coronaviruses Through Whole Genome Sequence Analysis.Viruses · 2020Article
- Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus.Virus research · 2020Article
- Prevalence of a novel bovine coronavirus strain with a recombinant hemagglutinin/esterase gene in dairy calves in China.Transboundary and emerging diseases · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spike glycoprotein is a major neutralizing antigen of bovine coronavirus (BCV). Conformational neutralizing epitopes of group A and group B monoclonal antibodies (MAbs) have previously been mapped to two domains at amino acids 351 to 403 (domain I) and amino acids 517 to 621 (domain II). To further map antigenic sites, neutralization escape mutants of BCV were selected with a group A MAb which has both in vitro and in vivo virus-neutralizing ability. The escape mutants were demonstrated to be neutralization resistant to the selecting group A MAb and remained sensitive to neutralization by a group B MAb. In radioimmunoprecipitation assays, the spike proteins of neutralization escape mutants were shown to have lost their reactivities with the selecting group A MAb. Sequence analysis of the spike protein genes of the escape mutants identified a single nucleotide substitution of C to T at position 1583, resulting in the change of alanine to valine at amino acid position 528 (A528V). The mutation occurs in domain II and in a location which corresponds to the hypervariable region of the spike protein of the coronavirus mouse hepatitis virus. Experimental introduction of the A528V mutation into the wild-type spike protein resulted in the loss of MAb binding of the mutant protein, confirming that the single point mutation was responsible for the escape of BCV from immunological selective pressure.
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