ArticleThe Biochemical journal2001
Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Two endogenousProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Diverse Roles of the Multiple Phosphodiesterases in the Regulation of Cyclic Nucleotide Signaling inCells · 2025Review
- Analysis of cGMP Signaling in Dictyostelium.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Forty-five years of cGMP research inMolecular biology of the cell · 2021Review
- Article
- An unusual and vital protein with guanylate cyclase and P4-ATPase domains in a pathogenic protist.Life science alliance · 2019Article
- An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.Molecular biology of the cell · 2019Article
- Mutational analysis gives insight into substrate preferences of a nucleotidyl cyclase from Mycobacterium avium.PloS one · 2014Article
- The prokaryotic origin and evolution of eukaryotic chemosignaling systems.Neuroscience and behavioral physiology · 2009Review
- The evolution of guanylyl cyclases as multidomain proteins: conserved features of kinase-cyclase domain fusions.Journal of molecular evolution · 2009Article
- Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells.Molecular biology of the cell · 2006Article
- Multiple lineage specific expansions within the guanylyl cyclase gene family.BMC evolutionary biology · 2006Article
- Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis.Molecular biology of the cell · 2005Article
- Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium.Molecular biology of the cell · 2002Article
- A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.The EMBO journal · 2002Article
- The ascent of nucleotide cyclases: conservation and evolution of a theme.Journal of biosciences · 2002Article
- Guanylyl cyclases in unicellular organisms.Molecular and cellular biochemistry · 2002Review
- The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The core of adenylate and guanylate cyclases is formed by an intramolecular or intermolecular dimer of two cyclase domains arranged in an antiparallel fashion. Metazoan membrane-bound adenylate cyclases are composed of 12 transmembrane spanning regions, and two cyclase domains which function as a heterodimer and are activated by G-proteins. In contrast, membrane-bound guanylate cyclases have only one transmembrane spanning region and one cyclase domain, and are activated by extracellular ligands to form a homodimer. In the cellular slime mould, Dictyostelium discoideum, membrane-bound guanylate cyclase activity is induced after cAMP stimulation; a G-protein-coupled cAMP receptor and G-proteins are essential for this activation. We have cloned a Dictyostelium gene, DdGCA, encoding a protein with 12 transmembrane spanning regions and two cyclase domains. Sequence alignment demonstrates that the two cyclase domains are transposed, relative to these domains in adenylate cyclases. DdGCA expressed in Dictyostelium exhibits high guanylate cyclase activity and no detectable adenylate cyclase activity. Deletion of the gene indicates that DdGCA is not essential for chemotaxis or osmo-regulation. The knock-out strain still exhibits substantial guanylate cyclase activity, demonstrating that Dictyostelium contains at least one other guanylate cyclase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.