ArticleThe Biochemical journal2001
Expression pattern and localization of beta,beta-carotene 15,15'-dioxygenase in different tissues.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 110 citations in OpenAlex.
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- Decoding color variation: genome-wide characterization and expression analysis of pigment pathways in Neocaridina denticulata.BMC genomics · 2026Article
- Retinoic acid production via the ray-finned fish genebioRxiv : the preprint server for biology · 2025Article
- Effect of Dietary Cholesterol, Phytosterol, and Docosahexaenoic Acid on Astaxanthin Absorption and Retention in Rainbow Trout.Aquaculture nutrition · 2024Article
- Molecular Properties of β-Carotene Oxygenases and Their Potential in Industrial Production of Vitamin A and Its Derivatives.Antioxidants (Basel, Switzerland) · 2022Review
- Beta-carotene and its protective effect on gastric cancer.World journal of clinical cases · 2021Review
- Carotenoid metabolism at the intestinal barrier.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Current State of Evidence: Influence of Nutritional and Nutrigenetic Factors on Immunity in the COVID-19 Pandemic Framework.Nutrients · 2020Review
- Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice.The Journal of biological chemistry · 2018Article
- RPE65 has an additional function as the lutein toProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Inhibition of pulmonary β-carotene 15, 15'-oxygenase expression by glucocorticoid involves PPARα.PloS one · 2017Article
- A genetic dissection of intestinal fat-soluble vitamin and carotenoid absorption.Human molecular genetics · 2015Article
- Cellular localization of β-carotene 15,15' oxygenase-1 (BCO1) and β-carotene 9',10' oxygenase-2 (BCO2) in rat liver and intestine.Archives of biochemistry and biophysics · 2015Article
- Actions of β-apo-carotenoids in differentiating cells: differential effects in P19 cells and 3T3-L1 adipocytes.Archives of biochemistry and biophysics · 2015Article
- Cardiac dysfunction in β-carotene-15,15'-dioxygenase-deficient mice is associated with altered retinoid and lipid metabolism.American journal of physiology. Heart and circulatory physiology · 2014Article
- The multifaceted nature of retinoid transport and metabolism.Hepatobiliary surgery and nutrition · 2014Review
- Enzymology of retinoic acid biosynthesis and degradation.Journal of lipid research · 2013Review
- Carotenoid metabolism in mammals, including man: formation, occurrence, and function of apocarotenoids.Journal of lipid research · 2013Review
- Genetics and diet regulate vitamin A production via the homeobox transcription factor ISX.The Journal of biological chemistry · 2013Article
- Lycopene metabolism and its biological significance.The American journal of clinical nutrition · 2012Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Beta,beta-carotene 15,15'-dioxygenase cleaves beta,beta-carotene into two molecules of retinal, and is the key enzyme in the metabolism of beta,beta-carotene to vitamin A. The enzyme has been known for more than 40 years, yet all attempts to purify the protein to homogeneity have failed. Recently, the successful cloning and sequencing of an enzyme with beta,beta-carotene 15,15'-dioxygenase activity from chicken, as well as from Drosophila, has been reported. Here, we describe in detail our attempt to enrich the chicken beta,beta-carotene 15,15'-dioxygenase to such an extent as to allow determination of partial amino acid sequences, which were then used to design degenerate oligonucleotides. Screening of a chicken duodenal expression library yielded a full-length clone containing a coding sequence of 1578 bp. Functional expression in Escherichia coli and in eukaryotic cell lines confirmed that we had cloned the first vertebrate dioxygenase that cleaves beta,beta-carotene at the central 15,15'-double bond. By performing a sequence homology search, the cDNA sequence of the mouse homologue was found as an expressed sequence tag (EST) in the gene bank. At the amino-acid level, the degree of homology between the chicken and mouse sequences is 81%. Thus beta,beta-carotene 15,15'-dioxygenase can be considered as being an enzyme that is evolutionarily rather well conserved. We established the expression pattern of beta,beta-carotene 15,15'-dioxygenase in chicken and mouse tissues with a combination of Northern blots and in situ hybridization. The mRNA for beta,beta-carotene 15,15'-dioxygenase was localized primarily in duodenal villi, as well as in liver and in tubular structures of lung and kidney. These new findings demonstrate that beta,beta-carotene 15,15'-dioxygenase is also expressed in epithelial structures, where it serves to provide the tissue-specific vitamin A supply.
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