ArticleJournal of clinical microbiology2001
Molecular characterization of fliD gene encoding flagellar cap and its expression among Clostridium difficile isolates from different serogroups.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Recombinant Fusion Protein Vaccine Containing Clostridioides difficile FliC and FliD Protects Mice against C. difficile Infection.Infection and immunity · 2023Article
- Bacteria with a mouth: Discovery and new insights into cell surface structure and macromolecule transport.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Article
- Immunoinformatics Approach Toward the Introduction of a Novel Multi-Epitope Vaccine AgainstFrontiers in immunology · 2022Article
- New ribotypeEmerging microbes & infections · 2021Article
- Identification of linear epitopes on the flagellar proteins of Clostridioides difficile.Scientific reports · 2021Article
- Isolation and characterization of a multidrug-resistantJournal of environmental health science & engineering · 2020Article
- Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi.Molecular microbiology · 2019Article
- Dual role of the colonization factor CD2831 in Clostridium difficile pathogenesis.Scientific reports · 2019Article
- Analysis of proteomes released from in vitro cultured eightGut pathogens · 2017Article
- Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli.Infection and immunity · 2016Article
- Immunogenicity and protective efficacy of recombinant Clostridium difficile flagellar protein FliC.Emerging microbes & infections · 2016Article
- Clostridium difficile virulence factors: Insights into an anaerobic spore-forming pathogen.Gut microbes · 2014Review
- The flagellin FliC of Clostridium difficile is responsible for pleiotropic gene regulation during in vivo infection.PloS one · 2014Article
- Immune-based treatment and prevention of Clostridium difficile infection.Human vaccines & immunotherapeutics · 2014Review
- The second messenger cyclic Di-GMP regulates Clostridium difficile toxin production by controlling expression of sigD.Journal of bacteriology · 2013Article
- Clostridium difficile infection in the twenty-first century.Emerging microbes & infections · 2013Review
- Identification of a novel zinc metalloprotease through a global analysis of Clostridium difficile extracellular proteins.PloS one · 2013Article
- The role of flagella in Clostridium difficile pathogenesis: comparison between a non-epidemic and an epidemic strain.PloS one · 2013Article
- Mutagenic analysis of the Clostridium difficile flagellar proteins, FliC and FliD, and their contribution to virulence in hamsters.Infection and immunity · 2011Article
- The key sigma factor of transition phase, SigH, controls sporulation, metabolism, and virulence factor expression in Clostridium difficile.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fliD gene encoding the flagellar cap protein (FliD) of Clostridium difficile was studied in 46 isolates belonging to serogroups A, B, C, D, F, G, H, I, K, X, and S3, including 30 flagellated strains and 16 nonflagellated strains. In all but three isolates, amplification by PCR and reverse transcription-PCR demonstrated that the fliD gene is present and transcribed in both flagellated and nonflagellated strains. PCR-restriction fragment length polymorphism (RFLP) analysis of amplified fliD gene products revealed interstrain homogeneity, with one of two major patterns (a and b) found in all but one of the strains, which had pattern c. A polyclonal monospecific antiserum raised to the recombinant FliD protein reacted in immunoblots with crude flagellar preparations from 28 of 30 flagellated strains but did not recognize FliD from nonflagellated strains. The fliD genes from five strains representative of the three different RFLP groups were sequenced, and sequencing revealed 100% identity between the strains with the same pattern and 88% identity among strains with different patterns. Our results show that even though FliD is a structure exposed to the outer environment, the flagellar cap protein is very well conserved, and this high degree of conservation suggests that it has a very specific function in attachment to cell or mucus receptors.
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