ArticleJournal of clinical microbiology2001
Antimicrobial resistance and bacterial identification utilizing a microelectronic chip array.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 80 citations in OpenAlex.
- Establishment of a Visual Gene Chip Method for the Simultaneous Detection of Seven Waterfowl Virus Pathogens.Viruses · 2025Article
- Bacterial capture by peptide-mimetic oligoacyllysine surfaces.Applied and environmental microbiology · 2010Article
- Current applications and future trends of molecular diagnostics in clinical bacteriology.Analytical and bioanalytical chemistry · 2009Review
- Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria.Journal of clinical microbiology · 2005Article
- Current and developing technologies for monitoring agents of bioterrorism and biowarfare.Clinical microbiology reviews · 2005Review
- env Gene typing of human immunodeficiency virus type 1 strains on electronic microarrays.Journal of clinical microbiology · 2005Article
- PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings.The Lancet. Infectious diseases · 2004Review
- Multiplex PCR, amplicon size and hybridization efficiency on the NanoChip electronic microarray.International journal of legal medicine · 2004Article
- Molecular diagnostics of clinically important staphylococci.Folia microbiologica · 2004Review
- gyrA polymorphism in Campylobacter jejuni: detection of gyrA mutations in 162 C. jejuni isolates by single-strand conformation polymorphism and DNA sequencing.Antimicrobial agents and chemotherapy · 2002Article
- DNA microarray-based typing of an atypical monophasic Salmonella enterica serovar.Journal of clinical microbiology · 2002Article
- Microarrays, antiobesity and the liver.Annals of hepatologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Species-specific bacterial identification of clinical specimens is often limited to a few species due to the difficulty of performing multiplex reactions. In addition, discrimination of amplicons is time-consuming and laborious, consisting of gel electrophoresis, probe hybridization, or sequencing technology. In order to simplify the process of bacterial identification, we combined anchored in situ amplification on a microelectronic chip array with discrimination and detection on the same platform. Here, we describe the simultaneous amplification and discrimination of six gene sequences which are representative of different bacterial identification assays: Escherichia coli gyrA, Salmonella gyrA, Campylobacter gyrA, E. coli parC, Staphylococcus mecA, and Chlamydia cryptic plasmid. The assay can detect both plasmid and transposon genes and can also discriminate strains carrying antibiotic resistance single-nucleotide polymorphism mutations. Finally, the assay is similarly capable of discriminating between bacterial species through reporter-specific discrimination and allele-specific amplification. Anchored strand displacement amplification allows multiplex amplification and complex genotype discrimination on the same platform. This assay simplifies the bacterial identification process greatly, allowing molecular biology techniques to be performed with minimal processing of samples and practical experience.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.