ArticleJournal of clinical microbiology2001
Isolation and characterization of polymorphic DNA from Entamoeba histolytica.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Investigating genetic polymorphism in E. histolytica isolates with distinct clinical phenotypes.Parasitology research · 2023Article
- Analysis of D-A locus of tRNA-linked short tandem repeats reveals transmission of Entamoeba histolytica and E. dispar among students in the Thai-Myanmar border region of northwest Thailand.PLoS neglected tropical diseases · 2021Article
- AIG1 affects in vitro and in vivo virulence in clinical isolates of Entamoeba histolytica.PLoS pathogens · 2018Article
- Article
- Genomic distribution of SINEs in Entamoeba histolytica strains: implication for genotyping.BMC genomics · 2013Article
- Unique short tandem repeat nucleotide sequences in Entamoeba histolytica isolates from China.Parasitology research · 2012Article
- Evidence for a link between locus R-R sequence type and outcome of infection with Entamoeba histolytica.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2012Article
- Polymorphism in two short tandem repeat loci (R-R and S-Q) linked to tRNA genes in Entamoeba dispar isolates.Gastroenterology and hepatology from bed to bench · 2012Article
- Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar.Iranian journal of parasitology · 2012Article
- Comparison of serine-rich protein genes of Entamoeba histolytica isolates obtained from institutions for the mentally retarded in Kanagawa and Shizuoka Prefectures, Japan.Parasitology research · 2010Article
- Human amebiasis: breaking the paradigm?International journal of environmental research and public health · 2010Article
- DNA characterization of simian Entamoeba histolytica-like strains to differentiate them from Entamoeba histolytica.Parasitology research · 2009Article
- Mutation detection analysis of a region of 16S-like ribosomal RNA gene of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii.BMC infectious diseases · 2008Article
- Molecular epidemiology of amebiasis.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2008Article
- Increased risk for Entamoeba histolytica infection and invasive amebiasis in HIV seropositive men who have sex with men in Taiwan.PLoS neglected tropical diseases · 2008Article
- Patterns of evolution in the unique tRNA gene arrays of the genus Entamoeba.Molecular biology and evolution · 2008Article
- Differences in protein profiles of the isolates of Entamoeba histolytica and E. dispar by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS) ProteinChip assays.Parasitology research · 2007Article
- Laboratory diagnostic techniques for Entamoeba species.Clinical microbiology reviews · 2007Review
- Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview.Experimental parasitology · 2007Review
- Prevalence and genetic diversity of Entamoeba histolytica in an institution for the mentally retarded in the Philippines.Parasitology research · 2006Article
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Authors and funding
2 authors.
Funding
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Abstract
An important gap in our understanding of the epidemiology of amebiasis is what determines the outcome of Entamoeba histolytica infections. To investigate the possible existence of invasive and noninvasive strains as one factor, the ability to differentiate individual isolates of E. histolytica is necessary. Two new loci containing internal repeats, locus 1-2 and locus 5-6, have been isolated. Each contains a single repeat block with two types of related direct repeats arranged in tandem. Southern blot analysis suggests that both loci are multicopy and may themselves be arranged in tandem arrays. Three other previously reported, internally repetitive loci containing at least two repeat blocks each with one or more related repeat units were also investigated. PCR was used to study polymorphism at each of these loci, which was detected to various degrees in each case. Variation was seen in the total number of bands obtained per isolate and their sizes. Nucleotide sequence comparison of loci 1-2 and 5-6 in five axenic isolates revealed differences in the number of repeat units, which correlated with the observed PCR product size variation, and in repeat sequence. Use of multiple loci collectively allowed differentiation of a majority of the 13 isolates studied, and we believe that these loci have the potential to be used as polymorphic molecular markers for investigating the epidemiology of E. histolytica and the potential existence of genetically distinct invasive and noninvasive strains.
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