ArticleGenome research2001
Fluorescence polarization in homogeneous nucleic acid analysis II: 5'-nuclease assay.
Article in Genome research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 61 citations in OpenAlex.
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- Fluorescence Polarization-Based Bioassays: New Horizons.Sensors (Basel, Switzerland) · 2020Review
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- A simple and rapid procedure for the detection of genes encoding Shiga toxins and other specific DNA sequences.Toxins · 2015Article
- Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
- Schistosome syntenin partially protects vaccinated mice against Schistosoma mansoni infection.PLoS neglected tropical diseases · 2014Article
- Functional characterization of ABCC2 promoter polymorphisms and allele-specific expression.The pharmacogenomics journal · 2013Article
- Enhanced degradation of dihydrofolate reductase through inhibition of NAD kinase by nicotinamide analogs.Molecular pharmacology · 2013Article
- Induction of heat shock proteins by hyperthermia and noise overstimulation in hsf1 -/- mice.Journal of the Association for Research in Otolaryngology : JARO · 2012Article
- A high-throughput method for the detection of homologous gene deletions in hexaploid wheat.BMC plant biology · 2010Article
- Identification of field caught Anopheles gambiae s.s. and Anopheles arabiensis by TaqMan single nucleotide polymorphism genotyping.Malaria journal · 2007Article
- Algorithm for automatic genotype calling of single nucleotide polymorphisms using the full course of TaqMan real-time data.Nucleic acids research · 2006Article
- Genotyping of single nucleotide substitutions.The Clinical biochemist. Reviews · 2006Article
- High-throughput SNP genotyping.Comparative and functional genomics · 2002Article
- Progress in high throughput SNP genotyping methods.The pharmacogenomics journal · 2002Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
When the temperature and viscosity of the solvent is held constant, the degree of fluorescence polarization (FP) detected when a fluorescent dye is excited by plane polarized light depends mostly on the molecular weight of the dye molecule. By monitoring the FP of a fluorescent dye molecule, one can detect significant changes in the molecular weight of a fluorescent molecule without separation or purification. The 5'-nuclease (TaqMan) assay is a robust single nucleotide polymorphism genotyping method where an allele-specific probe that binds to a perfectly complementary target is cleaved by the 5'-nuclease activity of Taq DNA polymerase. Because the TaqMan probe is labeled with a fluorescent dye, it has high FP value when intact but a low FP value after cleavage. In this study, we compared the results of the 5'-nuclease assay based on standard fluorescence intensity readings and FP readings when genotyping 90 individuals with 20 single nucleotide polymorphisms. Our results show that FP is just as robust and reliable as the standard fluorescence detection method. Use of FP detection makes it possible to reduce the cost of TaqMan probes by abrogating the need for a fluorescence quencher.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.