ArticleThe EMBO journal2001
Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 219 citations in OpenAlex.
- HOG Signaling: A Multifunctional Regulator of Stress Homeostasis inJournal of fungi (Basel, Switzerland) · 2026Review
- Dimorphism of oleaginous yeast Meyerozyma guilliermondii during single-cell oil production under decanoic acid stress.World journal of microbiology & biotechnology · 2026Article
- Bacterial metabolites induce cell wall remodeling, antifungal resistance, and immune recognition of commensal fungi.Current biology : CB · 2026Article
- N88S seipin-related seipinopathy is a lipidopathy associated with loss of iron homeostasis.Cell communication and signaling : CCS · 2025Article
- A shift in chromatin binding of phosphorylated p38 precedes transcriptional changes upon oxidative stress.FEBS letters · 2024Article
- Regulation of Catalase Expression and Activity byJournal of fungi (Basel, Switzerland) · 2024Article
- GCN4 Enhances the Transcriptional Regulation of AreA by Interacting with SKO1 To Mediate Nitrogen Utilization in Ganoderma lucidum.Applied and environmental microbiology · 2022Article
- The Transcription Factor CsAtf1 Negatively Regulates the Cytochrome P450 GeneJournal of fungi (Basel, Switzerland) · 2022Article
- Yeast osmoregulation - glycerol still in pole position.FEMS yeast research · 2022Review
- Data-driven identification of inherent features of eukaryotic stress-responsive genes.NAR genomics and bioinformatics · 2022Article
- Roles of High Osmolarity Glycerol and Cell Wall Integrity Pathways in Cadmium Toxicity inInternational journal of molecular sciences · 2021Article
- A self-balancing circuit centered on MoOsm1 kinase governs adaptive responses to host-derived ROS ineLife · 2020Article
- Yeast Ppz1 protein phosphatase toxicity involves the alteration of multiple cellular targets.Scientific reports · 2020Article
- Adaptive Laboratory Evolution and Reverse Engineering of Single-Vitamin Prototrophies in Saccharomyces cerevisiae.Applied and environmental microbiology · 2020Article
- A protein kinase A-regulated network encodes short- and long-lived cellular memories.Science signaling · 2020Article
- A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress.Nucleic acids research · 2020Article
- Accurate and Sensitive Quantitation of the Dynamic Heat Shock Proteome Using Tandem Mass Tags.Journal of proteome research · 2020Article
- Hog1-induced transcription of RTC3 and HSP12 is robust and occurs in cells lacking Msn2, Msn4, Hot1 and Sko1.PloS one · 2020Article
- Thiol-based direct threat sensing by the stress-activated protein kinase Hog1.Science signaling · 2019Article
- Deletion of theJournal of fungi (Basel, Switzerland) · 2019Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exposure of yeast to increases in extracellular osmolarity activates the Hog1 mitogen-activated protein kinase (MAPK), which is essential for the induction of gene expression required for cell survival upon osmotic stress. Several genes are regulated in response to osmotic stress by Sko1, a transcriptional repressor of the ATF/CREB family. We show by in vivo coprecipitation and phosphorylation studies that Sko1 and Hog1 interact and that Sko1 is phosphorylated upon osmotic stress in a Hog1-dependent manner. Hog1 phosphorylates Sko1 in vitro at multiple sites within the N-terminal region. Phosphorylation of Sko1 disrupts the Sko1-Ssn6-Tup1 repressor complex, and consistently, a mutant allele of Sko1, unphosphorylatable by Hog1, exhibits less derepression than the wild type. Interestingly, Sko1 repressor activity is further enhanced in strains with high protein kinase A (PKA) activity. PKA phosphorylates Sko1 near the bZIP domain and mutation of these sites eliminates modulation of Sko1 responses to high PKA activity. Thus, Sko1 transcriptional repression is controlled directly by the Hog1 MAPK in response to stress, and this effect is further modulated by an independent signaling mechanism through the PKA pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.