ArticleApplied and environmental microbiology2001
Molecular characterization of cryptosporidium oocysts in samples of raw surface water and wastewater.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers, 1 of them a synthesis that pooled it.
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Who cites it
128 citing papers in PubMed, 1 synthesis or guideline pooled it, 329 citations in OpenAlex.
- Prevalence of Cryptosporidium spp. in water: a global systematic review and meta-analysis.Environmental science and pollution research international · 2021Pooled it
- Prevalence, molecular species, and associated factors for Cryptosporidium infection among dairy-farm workers and HIV-positive individuals in central Ethiopia: a cross-sectional study.BMC infectious diseases · 2026Article
- Cryptosporidium geckonae n. sp. (Apicomplexa: Cryptosporidiidae) in geckos.Parasites & vectors · 2025Article
- Long-term molecular surveillance of Cryptosporidium and Giardia in wildlife in protected drinking water catchments.Parasites & vectors · 2025Article
- 18S/28S rDNA metabarcoding identifies Cryptosporidium parvum and Blastocystis ST1 as the predominant intestinal protozoa in hospital patients from Changchun, Northeast China.Parasites & vectors · 2025Article
- Cryptosporidium parvum multidrug resistance protein confers resistance to toxic gut microbial metabolite.Cell host & microbe · 2025Article
- Molecular identification and diversity of gastrointestinal apicomplexan protozoa in pigs in the Republic of Korea.Scientific reports · 2025Article
- Article
- Occurrence and Molecular Characterization of Cryptosporidium spp. and Giardia duodenalis in Water Buffaloes (Bubalis bubalis) From Türkiye.Acta parasitologica · 2025Article
- Cryptosporidium spp. in wild rodents in the Poyang Lake region, China.Parasite (Paris, France) · 2025Article
- Multicopy subtelomeric genes underlie animal infectivity of divergent Cryptosporidium hominis subtypes.Nature communications · 2024Article
- Wastewater-based intestinal protozoa monitoring in Shanghai, China.Microbiology spectrum · 2024Article
- Prevalence and genotypic characterization of zoonotic intestinal protozoan parasites in transboundary migratory Mongolian Gazelles (Parasitology · 2024Article
- Epidemiology and Molecular Characterization of Zoonotic Gastrointestinal Protozoal Infection in Zoo Animals in China.Animals : an open access journal from MDPI · 2024Article
- Molecular prevalence and associated risk factors ofVeterinary world · 2024Article
- First identification and coinfection detection of Enterocytozoon bieneusi, Encephalitozoon spp., Cryptosporidium spp. and Giardia duodenalis in diarrheic pigs in Southwest China.BMC microbiology · 2023Article
- Cryptosporidium mortiferum n. sp. (Apicomplexa: Cryptosporidiidae), the species causing lethal cryptosporidiosis in Eurasian red squirrels (Sciurus vulgaris).Parasites & vectors · 2023Article
- Prevalence and Molecular Characterization ofAnimals : an open access journal from MDPI · 2023Article
- Molecular identification and genetic characteristics of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in human immunodeficiency virus/acquired immunodeficiency syndrome patients in Shanghai, China.Parasites & vectors · 2023Article
- Multiple introductions and recombination events underlie the emergence of a hyper-transmissible Cryptosporidium hominis subtype in the USA.Cell host & microbe · 2023Article
68 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent molecular characterizations of Cryptosporidium parasites make it possible to differentiate the human-pathogenic Cryptosporidium parasites from those that do not infect humans and to track the source of Cryptosporidium oocyst contamination in the environment. In this study, we used a small-subunit rRNA-based PCR-restriction fragment length polymorphism (RFLP) technique to detect and characterize Cryptosporidium oocysts in 55 samples of raw surface water collected from several areas in the United States and 49 samples of raw wastewater collected from Milwaukee, Wis. Cryptosporidium parasites were detected in 25 surface water samples and 12 raw wastewater samples. C. parvum human and bovine genotypes were the dominant Cryptosporidium parasites in the surface water samples from sites where there was potential contamination by humans and cattle, whereas C. andersoni was the most common parasite in wastewater. There may be geographic differences in the distribution of Cryptosporidium genotypes in surface water. The PCR-RFLP technique can be a useful alternative method for detection and differentiation of Cryptosporidium parasites in water.
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