ArticleApplied and environmental microbiology2001
Protein trans-splicing to produce herbicide-resistant acetolactate synthase.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Seed- and leaf-based expression of FGF21-transferrin fusion proteins for oral delivery and treatment of non-alcoholic steatohepatitis.Frontiers in plant science · 2022Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- The application of gene splitting technique for controlling transgene flow in rice.Transgenic research · 2020Article
- EngineeredSaudi journal of biological sciences · 2019Article
- Intein applications: from protein purification and labeling to metabolic control methods.The Journal of biological chemistry · 2014Review
- Article
- Protein trans-splicing of multiple atypical split inteins engineered from natural inteins.PloS one · 2013Article
- Highly efficient and more general cis- and trans-splicing inteins through sequential directed evolution.The Journal of biological chemistry · 2011Article
- Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco.Applied and environmental microbiology · 2007Article
- Functional analysis of the split Synechocystis DnaE intein in plant tissues by biolistic particle bombardment.Transgenic research · 2006Article
- Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein splicing in trans has been demonstrated both in vivo and in vitro by biochemical and immunological analyses, but in vivo production of a functional protein by trans-splicing has not been reported previously. In this study, we used the DnaE intein from Synechocystis sp. strain PCC6803, which presumably reconstitutes functional DnaE protein by trans-splicing in vivo, to produce functional herbicide-resistant acetolactate synthase II (ALSII) from two unlinked gene fragments in Escherichia coli. The gene for herbicide-resistant ALSII was fused in frame to DnaE intein segments capable of promoting protein splicing in trans and was expressed from two compatible plasmids as two unlinked fragments. Cotransformation of E. coli with the two plasmids led to production of a functional enzyme that conferred herbicide resistance to the host E. coli cells. These results demonstrate the feasibility of expressing functional genes from two unlinked DNA loci and provide a model for the design of nontransferable transgenes in plants.
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