ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2001
Specific detection by PCR of Streptococcus agalactiae in milk.
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Molecular detection of Streptococcus Sp. isolated from the mouth and nose of humans and pets.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Isolation and characterization of Streptococcus agalactiae inducing mass mortalities in cultured Nile tilapia (Oreochromis niloticus) with trials for disease control using zinc oxide nanoparticles and ethanolic leaf extracts of some medicinal plants.BMC veterinary research · 2024Article
- Correlation of Streptococcus agalactiae concentration on immune system and effective dose of inactivated vaccine for Chitralada 3 strain Nile tilapia (Oreochromis niloticus) in Thailand.BMC veterinary research · 2023Article
- Effects of Bacillus spp. Mixture on Growth, Immune Responses, Expression of Immune-Related Genes, and Resistance of Nile Tilapia Against Streptococcus agalactiae Infection.Probiotics and antimicrobial proteins · 2023Article
- Do NAAT-Based Methods Increase the Diagnostic Sensitivity ofDiagnostics (Basel, Switzerland) · 2023Article
- Article
- Diversity ofFrontiers in veterinary science · 2023Article
- Isolation and molecular detection ofJournal of advanced veterinary and animal research · 2021Article
- Technological interventions and advances in the diagnosis of intramammary infections in animals with emphasis on bovine population-a review.The veterinary quarterly · 2019Review
- Molecular characterization of Streptococcus agalactiae isolated from bovine mastitis in Eastern China.PloS one · 2013Article
- A combined enrichment/polymerase chain reaction based method for the routine screening of Streptococcus agalactiae in pregnant women.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Molecular epidemiology of group B streptococcal infections.Folia microbiologica · 2004Review
- Serotypes of Streptococcus agalactiae cultured from dairy milk samples in Québec.The Canadian veterinary journal = La revue veterinaire canadienne · 2003Article
- Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens.Journal of clinical microbiology · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to develop a simple and specific method for direct detection of Streptococcus agalactiae from cow's milk. The method was based on polymerase chain reaction (PCR) using species-specific and universal primers derived from the 16S rRNA gene. The amplification product was verified by restriction endonuclease digest and sequencing. Specific identification was proven on a collection of 147 S. agalactiae isolates of bovine and human origin. In addition, 17 strains belonging to different bacterial species that potentially can be found in milk samples also tested negative. The PCR developed was used for direct detection of S. agalactiae in milk, using for the first time with gram-positive bacteria the nucleic acid-binding properties of diatomaceous earth. The test, which has high specificity, high sensitivity (100 cfu/mL), and can be carried out in less than 24 h, represents an innovative diagnostic tool for the detection of S. agalactiae in milk.
Indexed as
Identifiers
11227199PMC1189646What OpenQuestion holds
Registered trials
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