ArticleThe Journal of molecular diagnostics : JMD2001
Polymerase chain reaction detection of Kaposi's sarcoma-associated herpesvirus-optimized protocols and their application to myeloma.
Article in The Journal of molecular diagnostics : JMD, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 71 citations in OpenAlex.
- Recent Advances in the Histopathology, Molecular Biology, and Treatment of Kaposi Sarcoma: A Contemporary Review.International journal of molecular sciences · 2025Review
- Viral Load Measurements for Kaposi Sarcoma Herpesvirus (KSHV/HHV8): Review and an Updated Assay.Journal of medical virology · 2024Review
- Rapid, equipment-free extraction of DNA from skin biopsies for point-of-care diagnostics.Scientific reports · 2024Article
- Human herpesvirus-8 infection in Tunisian adult acute leukemia patients.African health sciences · 2023Article
- The First Evidence ofFrontiers in cellular and infection microbiology · 2019Article
- Current status of treatment for primary effusion lymphoma.Intractable & rare diseases research · 2014Review
- Association of Kaposi’s sarcoma-associated herpesvirus (KSHV) with bladder cancer in Croatian patients.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Characterization of human herpes virus 8 genotypes in Kaposi's sarcoma patients in Tehran, Iran.International journal of molecular epidemiology and genetics · 2012Article
- Detection of viral DNA sequences in sporadic colorectal cancers in relation to CpG island methylation and methylator phenotype.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- Oral hairy leukoplakia which occurred as a presenting sign of acute myeloid leukemia in a child.Annals of dermatology · 2010Article
- Immunohistochemical study association between human herpesvirus 8 and multiple myeloma.International journal of hematology · 2008Article
- Reflections on the interpretation of heterogeneity and strain differences based on very limited PCR sequence data from Kaposi's sarcoma-associated herpesvirus genomes.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- Investigating the presence of human herpesvirus 7 and 8 in multiple sclerosis and normal control brain tissue.Journal of the neurological sciences · 2006Article
- KSHV/HHV-8 associated lymph node based lymphomas in HIV seronegative subjects. Report of two cases with anaplastic large cell morphology and plasmablastic immunophenotype.Journal of clinical pathology · 2005Article
- Possible role of human herpesvirus 8 in the lymphoproliferative disorders in common variable immunodeficiency.The Journal of experimental medicine · 2005Article
- Kaposi's sarcoma-associated herpesvirus/human herpesvirus type 8-positive solid lymphomas: a tissue-based variant of primary effusion lymphoma.The Journal of molecular diagnostics : JMD · 2005Article
- Qualitative and quantitative analysis of human herpesviruses in chronic and acute B cell lymphocytic leukemia and in multiple myeloma.Leukemia · 2003Article
- Laboratory diagnosis of human herpesvirus 8 infection in humans.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2002Review
- Human herpesvirus 8 (HHV-8) detected in two patients with Kaposi's sarcoma-like pyogenic granuloma.Journal of clinical pathology · 2002Article
- Spectrum of Kaposi's sarcoma-associated herpesvirus, or human herpesvirus 8, diseases.Clinical microbiology reviews · 2002Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since its discovery in 1994, KSHV (also called human herpesvirus-8 or HHV8) has been implicated in a variety of disorders. Although the association of KSHV with Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman's disease has been well established, its presence in some other diseases, such as multiple myeloma, remains controversial. Because most KSHV studies are based on polymerase chain reaction (PCR) analysis, the conflicting data may be attributable to variations in the methods, primer sets, and target sequences selected. To establish an efficient and reliable PCR approach for KSHV detection we designed eight sets of primers to six regions (ORFK1, ORFK2, ORFK9, ORK26, ORF72, and ORF74) of the KSHV genome using appropriate database and software. The detection sensitivity of these primers was carefully assessed and their reliability was strictly validated in a series of positive (15 KS and PEL samples) and negative (16 lymphoid tissues) controls. We found that primer sets to the ORFK9 region showed the highest sensitivity, whereas primer sets to ORFK1 and ORF74 showed the lowest sensitivity. Primer sets to ORFK9, ORF26 and ORF72 regions detected all of the positive cases, whereas other primer sets showed varying detection rates or nonspecific bands. All 16 negative controls were negative with all primer sets. However, six of 16 negative controls became positive when we used nested PCR targeting ORF26. Therefore, multiple target KSHV sequences increase the detection efficiency, while nested PCR protocols are likely to introduce false positivity. Using ORFK9, ORF26 and ORF72 primer sets, we screened bone marrow biopsies from 18 cases of multiple myeloma, and failed to detect any KSHV sequences. This finding supports the conclusion that KSHV is not associated with multiple myeloma. Indeed, our results further confirm that although KSHV is universally present in Kaposi's sarcoma and primary effusion lymphoma, it is not ubiquitious.
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