ArticleProceedings of the National Academy of Sciences of the United States of America2001
Establishment of medaka (Oryzias latipes) transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells: a useful model to monitor germ cells in a live vertebrate.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 179 citations in OpenAlex.
- Transcriptomic and epigenetic alterations in aging-related genes in the medaka liver due to ancestral BPA exposure.Journal of the Endocrine Society · 2026Article
- Cyprinid Juji (Gobiocypris rarus) as a model fish to study germ cell development and gonadal differentiation.Communications biology · 2025Article
- Deciphering the gonadal cell atlas of Monopterus albus and cell fate during sex reversal based on single-cell RNA sequencing.Stem cell research & therapy · 2025Article
- Highly efficient transgenesis mediated by Tip100 transposon system in medaka.Transgenic research · 2025Article
- Article
- Stable Transmission of DNA Methylation Epimutations from Germlines to the Liver and Their Association with Fatty Liver Disease in Medaka.Research square · 2025Article
- Migration of primordial germ cells and their relationship of PGCs with sex development in transgenic germline-specific fluorescent freshwater angelfish (Pterophyllum scalare).Scientific reports · 2025Article
- Sex-dependent regulation of vertebrate somatic growth and aging by germ cells.Science advances · 2024Article
- The PCOS-NAFLD Multidisease Phenotype Occurred in Medaka Fish Four Generations after the Removal of Bisphenol A Exposure.Environmental science & technology · 2023Article
- Role and relevance of fish cell lines in advanced in vitro research.Molecular biology reports · 2022Review
- Article
- Dynamic transcriptional and chromatin accessibility landscape of medaka embryogenesis.Genome research · 2020Article
- The dynamics of DNA methylation during epigenetic reprogramming of primordial germ cells in medaka (Epigenetics · 2020Article
- Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon.Biomolecules · 2019Article
- A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka.PLoS biology · 2019Article
- Contrasting patterns of molecular evolution in metazoan germ line genes.BMC evolutionary biology · 2019Article
- Enhanced Adhesion of Fish Ovarian Germline Stem Cells on Solid Surfaces by Mussel-Inspired Polymer Coating.Marine drugs · 2018Article
- The Power of Fish Models to Elucidate Skin Cancer Pathogenesis and Impact the Discovery of New Therapeutic Opportunities.International journal of molecular sciences · 2018Review
- Germ cells in the teleost fish medaka have an inherent feminizing effect.PLoS genetics · 2018Article
- Black carp vasa identifies embryonic and gonadal germ cells.Development genes and evolution · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have generated transgenic medaka (teleost, Oryzias latipes), which allow us to monitor germ cells by green fluorescent protein (GFP) fluorescence in live specimens. Two medaka strains, himedaka (orange-red variety) and inbred QurtE, were used. The transgenic lines were achieved by microinjection of a construct containing the putative promoter region and 3' region of the medaka vasa gene (olvas). The intensity of GFP fluorescence increases dramatically in primordial germ cells (PGCs) located in the ventrolateral region of the posterior intestine around stage 25 (the onset of blood circulation). Whole-mount in situ hybridization and monitoring of ectopically located cells by GFP fluorescence suggested that (i) the increase in zygotic olvas expression occurs after PGC specification and (ii) PGCs can maintain their cell characteristics ectopically after stages 20-25. Around the day of hatching, the QurtE strain clearly exhibits sexual dimorphisms in the number of GFP fluorescent germ cells, a finding consistent with the appearance of leucophores, a sex-specific marker of QurtE. The GFP expression persists throughout the later stages in the mature ovary and testis. Thus, these transgenic medaka represent a live vertebrate model to investigate how germ cells migrate to form sexually dimorphic gonads, as well as a potential assay system for environmental substances that may affect gonad development. The use of a transgenic construct as a selective marker to efficiently isolate germ-line-transmitting founders during embryogenesis is also discussed.
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