ArticleProceedings of the National Academy of Sciences of the United States of America2001
Production of zebrafish germ-line chimeras from embryo cell cultures.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 162 citations in OpenAlex.
- Emerging Frontiers in Zebrafish Embryonic and Adult-Derived Cell Lines.International journal of molecular sciences · 2025Review
- Established and emerging new approach methodologies in neuroscience.Frontiers in neuroscience · 2025Review
- Distinguishing between ante factum and post factum properties of animal cell lines and demonstrating their use in grouping ray-finned fish cell lines into invitromes.In vitro cellular & developmental biology. Animal · 2023Review
- The herbal extract deriving from aerial parts of Scutellaria baicalensis shows anti-inflammation and anti-hypoxia responses in cultured fin cells from rabbit fish.Fish & shellfish immunology · 2020Article
- Establishment and characterization of a brain cell line from sea perch, Lateolabrax japonicus.In vitro cellular & developmental biology. Animal · 2017Article
- Black carp vasa identifies embryonic and gonadal germ cells.Development genes and evolution · 2017Article
- Cryobanking of aquatic species.Aquaculture (Amsterdam, Netherlands) · 2017Article
- Interplay of Regulatory T Cell and Th17 Cells during Infectious Diseases in Humans and Animals.Frontiers in immunology · 2017Review
- Efficient transgenesis mediated by pigmentation rescue in zebrafish.BioTechniques · 2016Article
- Derivation and long-term culture of an embryonic stem cell-like line from zebrafish blastomeres under feeder-free condition.Zebrafish · 2014Article
- Deriving cell lines from zebrafish embryos and tumors.Zebrafish · 2013Article
- Article
- Interordinal chimera formation between medaka and zebrafish for analyzing stem cell differentiation.Stem cells and development · 2012Article
- Study of pluripotency markers in zebrafish embryos and transient embryonic stem cell cultures.Zebrafish · 2011Article
- Tumor suppressor Lzap regulates cell cycle progression, doming, and zebrafish epiboly.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Article
- Identification of pluripotency genes in the fish medaka.International journal of biological sciences · 2011Article
- Fish stem cell cultures.International journal of biological sciences · 2011Review
- Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos.Cellular and molecular life sciences : CMLS · 2010Article
- Somatic cell nuclear transfer in zebrafish.Nature methods · 2009Article
- Zebrafish primordial germ cell cultures derived from vasa::RFP transgenic embryos.Stem cells and development · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the zebrafish possesses many characteristics that make it a valuable model for genetic studies of vertebrate development, one deficiency of this model system is the absence of methods for cell-mediated gene transfer and targeted gene inactivation. In mice, embryonic stem cell cultures are routinely used for gene transfer and provide the advantage of in vitro selection for rare events such as homologous recombination and targeted mutation. Transgenic animals possessing a mutated copy of the targeted gene are generated when the selected cells contribute to the germ line of a chimeric embryo. Although zebrafish embryo cell cultures that exhibit characteristics of embryonic stem cells have been described, successful contribution of the cells to the germ-cell lineage of a host embryo has not been reported. In this study, we demonstrate that short-term zebrafish embryo cell cultures maintained in the presence of cells from a rainbow trout spleen cell line (RTS34st) are able to produce germ-line chimeras when introduced into a host embryo. Messenger RNA encoding the primordial germ-cell marker, vasa, was present for more than 30 days in embryo cells cocultured with RTS34st cells or their conditioned medium and disappeared by 5 days in the absence of the spleen cells. The RTS34st cells also inhibited melanocyte and neuronal cell differentiation in the embryo cell cultures. These results suggest that the RTS34st splenic-stromal cell line will be a valuable tool in the development of a cell-based gene transfer approach to targeted gene inactivation in zebrafish.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.