ArticleThe Plant cell2001
Cytogenomic analyses reveal the structural plasticity of the chloroplast genome in higher plants.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Comparative analysis of morphology and chloroplast genomes in endangered plantFrontiers in plant science · 2026Article
- Characterization of the complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2026Article
- Sequencing, Assembly, and Comparative Evolutionary Analysis of the Chloroplast Genome of Kenaf (Genes · 2025Article
- Complete Chloroplast Genome Characterization, and Phylogenetic Analyses of the Rare and Endangered PlantBiology · 2025Article
- Comparative pan-genome analysis of Huperzia and Phlegmariurus and transcriptomics reveals thermal adaptation in Huperzia.Functional & integrative genomics · 2025Article
- The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2025Article
- The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2025Article
- Article
- The Complete Chloroplast Genome ofGenes · 2024Article
- Highly active repeat-mediated recombination in the mitogenome of the aquatic grass Hygroryza aristata.BMC plant biology · 2024Article
- A novel in-situ-process technique constructs whole circular cpDNA library.Plant methods · 2024Article
- The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2024Article
- More than a spiny morphology: plastome variation in the prickly pear cacti (Opuntieae).Annals of botany · 2023Article
- Extrachromosomal circular DNA-mediated spread of herbicide resistance in interspecific hybrids of pigweed.Plant physiology · 2023Article
- Article
- Plastid Genomes of Flowering Plants: Essential Principles.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Comparative analysis of chloroplast genomes in Vasconcellea pubescens A.DC. and Carica papaya L.Scientific reports · 2020Article
- GetOrganelle: a fast and versatile toolkit for accurate de novo assembly of organelle genomes.Genome biology · 2020Article
- Unprecedented Intraindividual Structural Heteroplasmy in Eleocharis (Cyperaceae, Poales) Plastomes.Genome biology and evolution · 2020Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A DNA fiber-based fluorescence in situ hybridization (fiber-FISH) technique was developed to analyze the structure and organization of a large number of intact chloroplast DNA (cpDNA) molecules from Arabidopsis, tobacco, and pea. Using this cytogenomic approach, we determined that 25 to 45% of the cpDNA within developing leaf tissue consists of circular molecules. Both linear and circular DNA fibers with one to four copies of the chloroplast genome were present, with monomers being the predominant structure. Arabidopsis and tobacco chloroplasts contained previously unidentified multimers (>900 kb) consisting of six to 10 genome equivalents. We further discovered rearranged cpDNA molecules of incomplete genome equivalents, confirmed by both differential hybridizations and size estimations. The unique cpDNA organization and novel structures revealed in this study demonstrate that higher plant cpDNA is more structurally plastic than previous sequence and electrophoretic analyses have suggested. Additionally, we demonstrate how the fiber-FISH-based cytogenomic approach allows for powerful analysis of very rare events that cannot be detected by traditional techniques such as DNA gel blot hybridization or polymerase chain reaction.
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