ArticleThe EMBO journal2001
A thermostable platform for transcriptional regulation: the DNA-binding properties of two Lrp homologs from the hyperthermophilic archaeon Methanococcus jannaschii.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Analysis ofGenes · 2021Article
- CopR, a Global Regulator of Transcription to Maintain Copper Homeostasis inFrontiers in microbiology · 2020Article
- Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures inPeerJ · 2019Article
- The interplay between nucleoid organization and transcription in archaeal genomes.Nature reviews. Microbiology · 2015Review
- Evolution of context dependent regulation by expansion of feast/famine regulatory proteins.BMC systems biology · 2014Article
- Article
- The genome-wide binding profile of the Sulfolobus solfataricus transcription factor Ss-LrpB shows binding events beyond direct transcription regulation.BMC genomics · 2013Article
- The Lrp family of transcription regulators in archaea.Archaea (Vancouver, B.C.) · 2010Article
- Transcriptional control by two leucine-responsive regulatory proteins in Halobacterium salinarum R1.BMC molecular biology · 2010Article
- Transcriptional activation in the context of repression mediated by archaeal histones.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The DNA-recognition mode shared by archaeal feast/famine-regulatory proteins revealed by the DNA-binding specificities of TvFL3, FL10, FL11 and Ss-LrpB.Nucleic acids research · 2009Article
- Protein-coding gene promoters in Methanocaldococcus (Methanococcus) jannaschii.Nucleic acids research · 2009Article
- A whole-genome approach to identifying protein binding sites: promoters in Methanocaldococcus (Methanococcus) jannaschii.Nucleic acids research · 2008Article
- Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing.Nucleic acids research · 2007Article
- Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of Ss-LrpB, a transcription regulator from Sulfolobus solfataricus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2005Article
- An expanding family of archaeal transcriptional activators.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The archaeal feast/famine regulatory protein: potential roles of its assembly forms for regulating transcription.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Activation of archaeal transcription by recruitment of the TATA-binding protein.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A Pyrococcus homolog of the leucine-responsive regulatory protein, LrpA, inhibits transcription by abrogating RNA polymerase recruitment.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, related to the bacterial Lrp/AsnC family of transcriptional regulators. We show that these two small helix-turn-helix proteins are specific DNA-binding proteins recognizing sites in their respective promoter regions. In vitro selection at high temperature has been used to isolate sets of high- affinity DNA sites that define a palindromic consensus binding sequence for each protein. Ptr1 and Ptr2 bind these cognate sites from one side of the DNA helix, as dimers, with each protein monomer making base- specific contacts in the major groove. As the first archaeal DNA-binding proteins with clearly defined specificities, Ptr1 and Ptr2 provide a thermostable DNA-binding platform for analysis of effector interactions with the core archaeal transcription apparatus; a platform allowing manipulation of promoter structure and examination of mechanisms of action at heterologous promoters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.